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Analytical Tools In Biotechnology — Part 1 MCQs with Explanations

Practice high-yield Biotechnology Engineering MCQs on Analytical Tools In Biotechnology — Part 1 with worked academic solutions for PPSC, FPSC, NIH, NTS.

Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40

Verified Practice Questions

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Question 1 of 40
★ PPSC ★ FPSC

Which of the following could be used as the layer of dielectric in interference filters used in Absorption Spectroscopy?

Question 2 of 40
★ PPSC ★ FPSC

In Atomic Absorption Spectroscopy, with what material is the cathode in Hollow cathode lamp constructed?

Question 3 of 40
★ PPSC ★ FPSC

Atomic absorption spectroscopy is also called as Absorption Flame Photometry.

Question 4 of 40
★ PPSC ★ FPSC

Transmittance is given as $$T = \frac{P}{{{P_o}}}.$$ If Po is the power incident on the sample, what does P represent?

Question 5 of 40
★ PPSC ★ FPSC

How is the wave number of electromagnetic radiation related to wavelength?

Question 6 of 40
★ PPSC ★ FPSC

In Flame emission photometers, the measurement of . . . . . . . . is used for qualitative analysis.

Question 7 of 40
★ PPSC ★ FPSC

What is the unit of absorbance which can be derived from Beer Lambert's law?

Question 8 of 40
★ PPSC ★ FPSC

Match the items in Group I with Group II . Group I Group II P. Circular dichroism 1. Concentration Q. X-ray crystallography 2. Sedimentation coefficient R. Freeze-drying 3. Secondary structure determination S. Ultracentrifugation 4. Tertiary structure determination

Question 9 of 40
★ PPSC ★ FPSC

Bolometer, a type of detector, is also known as . . . . . . . .

Question 10 of 40
★ PPSC ★ FPSC

To tolerate high operating temperatures, which of the following has to be done in incandescent or tungsten filament lamps?

Question 11 of 40
★ PPSC ★ FPSC

The helix content of a protein can be determined using

Question 12 of 40
★ PPSC ★ FPSC

How can the intensity of radiation be increased in Hollow cathode lamp?

Question 13 of 40
★ PPSC ★ FPSC

Tertiary structure of a protein consisting of α-helices and β-strands can be determined by

Question 14 of 40
★ PPSC ★ FPSC

Which of the following is the advantage of grating monochromators?

Question 15 of 40
★ PPSC ★ FPSC

In Atomic Absorption Spectroscopy, which of the following is the generally used radiation source?

Question 16 of 40
★ PPSC ★ FPSC

Which of the following is not a technique for preparing solid samples in IR spectroscopy?

Question 17 of 40
★ PPSC ★ FPSC

Which of the following options are correct in terms of wavelength for the different types of IR spectrometer?

Question 18 of 40
★ PPSC ★ FPSC

Which of the following is the principle of Atomic Absorption Spectroscopy?

Question 19 of 40
★ PPSC ★ FPSC

In the most widely used beam splitter, a thin film of . . . . . . . . is sandwiched between two plates of low refractive index solid. Fill the blank with a suitable option.

Question 20 of 40
★ PPSC ★ FPSC

Laminar flow burner used in Flame photometers is also known as . . . . . . . .

Question 21 of 40
★ PPSC ★ FPSC

Which of the following is not the advantage of Fourier Transform Spectrometers?

Question 22 of 40
★ PPSC ★ FPSC

Which of the following is not used as pyroelectric material used in pyroelectric transducers in Infrared spectroscopy?

Question 23 of 40
★ PPSC ★ FPSC

In Michelson's interferometer, the . . . . . . . . of the detector output will depend upon the intensity of incoming radiation.

Question 24 of 40
★ PPSC ★ FPSC

Which of the following statement is false about double beam absorption instruments?

Question 25 of 40
★ PPSC ★ FPSC

Which of the following is false about the wavelengths of electromagnetic radiation?

Question 26 of 40
★ PPSC ★ FPSC

In Michelson Interferometer, if the reflected and transmitted beams are in phase at the beam splitter, then maximum intensity will reach the detector.

Question 27 of 40
★ PPSC ★ FPSC

Which of the following is the purpose of the beam splitter in double beam photometer or colorimeter?

Question 28 of 40
★ PPSC ★ FPSC

Only pyroelectric transducer or pyroelectric crystals are used as detectors in Fourier Transform Infrared Spectrophotometer (FTIR). What is the main reason for other types of thermal detectors are not being used in FTIR spectrophotometer?

Question 29 of 40
★ PPSC ★ FPSC

What is the unit of molar absorptivity or absorptivity which is used to determine absorbance A in Beer Lambert's formula?

Question 30 of 40
★ PPSC ★ FPSC

In Michelson's interferometer, the frequency of the detector output can be determined by translating the . . . . . . . . of movable mirror and the . . . . . . . . of monochromatic radiation.

Question 31 of 40
★ PPSC ★ FPSC

The function of pressure regulators in the emission system of flame photometer is to have a steady flame which is free from flickers.

Question 32 of 40
★ PPSC ★ FPSC

Colorimeters are used in applications where great accuracy is required.

Question 33 of 40
★ PPSC ★ FPSC

Which of the following is not an application of Flame emission photometers?

Question 34 of 40
★ PPSC ★ FPSC

Enzyme-linked immunosorbent assay (ELISA) is used for

Question 35 of 40
★ PPSC ★ FPSC

How is Tungsten Halogen lamp differs from normal Tungsten filament lamp used in absorption spectroscopy?

Question 36 of 40
★ PPSC ★ FPSC

Which of the following detectors does not require a battery and is also known as barrier layer cell?

Question 37 of 40
★ PPSC ★ FPSC

Which of the following is not true about Absorption spectroscopy?

Question 38 of 40
★ PPSC ★ FPSC

In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with

Question 39 of 40
★ PPSC ★ FPSC

Which of the following is the principle of Golay cell which is used as a detector in IR spectroscopy?

Question 40 of 40
★ PPSC ★ FPSC

Group I lists spectroscopic methods and Group II lists bimolecular applications of these methods. Match the methods in Group I with the applications in Group II . Group I Group II P. Infrared 1. Identification of functional groups Q. Circular Dichroism 2. Determination of secondary structure R. Nuclear Magnetic Resonance 3. Estimation of molecular weight 4. Determination of 3-D structure

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Analytical Tools In Biotechnology — Part 2

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💡 Examiner's Advisory for Biotechnology Engineering

In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.

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