Analytical Tools In Biotechnology — Part 1 MCQs with Explanations
Practice high-yield Biotechnology Engineering MCQs on Analytical Tools In Biotechnology — Part 1 with worked academic solutions for PPSC, FPSC, NIH, NTS.
Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40
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Question 1 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following could be used as the layer of dielectric in interference filters used in Absorption Spectroscopy?
Correct Answer: Option B — MgF2
Detailed Academic Explanation:
MgF2 is the correct answer to “Which of the following could be used as the layer of dielectric in interference filters used in Absorption Spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 2 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In Atomic Absorption Spectroscopy, with what material is the cathode in Hollow cathode lamp constructed?
Correct Answer: Option C — Element to be investigated
Detailed Academic Explanation:
Element to be investigated is the correct answer to “In Atomic Absorption Spectroscopy, with what material is the cathode in Hollow cathode lamp constructed”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 3 of 40
★ PPSC
★ FPSC
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Atomic absorption spectroscopy is also called as Absorption Flame Photometry.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “Atomic absorption spectroscopy is also called as Absorption Flame Photometry.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 4 of 40
★ PPSC
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Transmittance is given as $$T = \frac{P}{{{P_o}}}.$$ If Po is the power incident on the sample, what does P represent?
Correct Answer: Option A — Radiant power transmitted by the sample
Detailed Academic Explanation:
Radiant power transmitted by the sample is the correct answer to “Transmittance is given as $$T = \frac{P}{{{P_o}}}.$$ If Po is the power incident on the sample, what does P represent”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 5 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
How is the wave number of electromagnetic radiation related to wavelength?
Correct Answer: Option A — It is the reciprocal of wavelength
Detailed Academic Explanation:
It is the reciprocal of wavelength is the correct answer to “How is the wave number of electromagnetic radiation related to wavelength”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 6 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In Flame emission photometers, the measurement of . . . . . . . . is used for qualitative analysis.
Correct Answer: Option A — Colour
Detailed Academic Explanation:
Colour is the correct answer to “In Flame emission photometers, the measurement of . . . . . . . . is used for qualitative analysis.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 7 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
What is the unit of absorbance which can be derived from Beer Lambert's law?
Correct Answer: Option D — No unit
Detailed Academic Explanation:
No unit is the correct answer to “What is the unit of absorbance which can be derived from Beer Lambert's law”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 8 of 40
★ PPSC
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Match the items in Group I with Group II . Group I Group II P. Circular dichroism 1. Concentration Q. X-ray crystallography 2. Sedimentation coefficient R. Freeze-drying 3. Secondary structure determination S. Ultracentrifugation 4. Tertiary structure determination
Correct Answer: Option D — P-3, Q-4, R-1, S-2
Detailed Academic Explanation:
P-3, Q-4, R-1, S-2 is the correct answer to “Match the items in Group I with Group II . Group I Group II P. Circular dichroism 1. Concentration Q. X-ray crystallography 2. Sedimentation coefficient R. Freeze-drying 3. Secondary structure determination S. Ultracentrifugation 4. Tertiary structure determination”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 9 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Bolometer, a type of detector, is also known as . . . . . . . .
Correct Answer: Option B — Thermistor
Detailed Academic Explanation:
Thermistor is the correct answer to “Bolometer, a type of detector, is also known as . . . . . . . .”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 10 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
To tolerate high operating temperatures, which of the following has to be done in incandescent or tungsten filament lamps?
Correct Answer: Option C — Envelope is fabricated with quartz
Detailed Academic Explanation:
Envelope is fabricated with quartz is the correct answer to “To tolerate high operating temperatures, which of the following has to be done in incandescent or tungsten filament lamps”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 11 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The helix content of a protein can be determined using
Correct Answer: Option C — A circular dichroism spectrometer
Detailed Academic Explanation:
A circular dichroism spectrometer is the correct answer to “The helix content of a protein can be determined using”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 12 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
How can the intensity of radiation be increased in Hollow cathode lamp?
Correct Answer: Option A — Addition of non-conductive protective shield of mica
Detailed Academic Explanation:
Addition of non-conductive protective shield of mica is the correct answer to “How can the intensity of radiation be increased in Hollow cathode lamp”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 13 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Tertiary structure of a protein consisting of α-helices and β-strands can be determined by
Correct Answer: Option C — Nuclear magnetic resonance spectroscopy
Detailed Academic Explanation:
Nuclear magnetic resonance spectroscopy is the correct answer to “Tertiary structure of a protein consisting of α-helices and β-strands can be determined by”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 14 of 40
★ PPSC
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★ FPSC
Which of the following is the advantage of grating monochromators?
Correct Answer: Option D — Dispersion occurs in a linear manner
Detailed Academic Explanation:
Dispersion occurs in a linear manner is the correct answer to “Which of the following is the advantage of grating monochromators”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 15 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In Atomic Absorption Spectroscopy, which of the following is the generally used radiation source?
Correct Answer: Option D — Hollow cathode lamp
Detailed Academic Explanation:
Hollow cathode lamp is the correct answer to “In Atomic Absorption Spectroscopy, which of the following is the generally used radiation source”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 16 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a technique for preparing solid samples in IR spectroscopy?
Correct Answer: Option D — Thin films
Detailed Academic Explanation:
Thin films is the correct answer to “Which of the following is not a technique for preparing solid samples in IR spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 17 of 40
★ PPSC
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Which of the following options are correct in terms of wavelength for the different types of IR spectrometer?
Correct Answer: Option A — Near IR: 0.8 - 2.5 mm
Detailed Academic Explanation:
Near IR: 0.8 - 2.5 mm is the correct answer to “Which of the following options are correct in terms of wavelength for the different types of IR spectrometer”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 18 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the principle of Atomic Absorption Spectroscopy?
Correct Answer: Option A — Radiation is absorbed by non-excited atoms in vapour state and are excited to higher states
Detailed Academic Explanation:
Radiation is absorbed by non-excited atoms in vapour state and are excited to higher states is the correct answer to “Which of the following is the principle of Atomic Absorption Spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 19 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In the most widely used beam splitter, a thin film of . . . . . . . . is sandwiched between two plates of low refractive index solid. Fill the blank with a suitable option.
Correct Answer: Option A — Mylar
Detailed Academic Explanation:
Mylar is the correct answer to “In the most widely used beam splitter, a thin film of . . . . . . . . is sandwiched between two plates of low refractive index solid. Fill the blank with a suitable option.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 20 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Laminar flow burner used in Flame photometers is also known as . . . . . . . .
Correct Answer: Option B — Premix burner
Detailed Academic Explanation:
Premix burner is the correct answer to “Laminar flow burner used in Flame photometers is also known as . . . . . . . .”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 21 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not the advantage of Fourier Transform Spectrometers?
Correct Answer: Option D — Easy to maintain
Detailed Academic Explanation:
Easy to maintain is the correct answer to “Which of the following is not the advantage of Fourier Transform Spectrometers”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 22 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not used as pyroelectric material used in pyroelectric transducers in Infrared spectroscopy?
Correct Answer: Option D — Tetraglycine sulphate
Detailed Academic Explanation:
Tetraglycine sulphate is the correct answer to “Which of the following is not used as pyroelectric material used in pyroelectric transducers in Infrared spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 23 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In Michelson's interferometer, the . . . . . . . . of the detector output will depend upon the intensity of incoming radiation.
Correct Answer: Option C — Amplitude
Detailed Academic Explanation:
Amplitude is the correct answer to “In Michelson's interferometer, the . . . . . . . . of the detector output will depend upon the intensity of incoming radiation.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 24 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following statement is false about double beam absorption instruments?
Correct Answer: Option C — Reference beam must have a higher intensity than sample beam
Detailed Academic Explanation:
Reference beam must have a higher intensity than sample beam is the correct answer to “Which of the following statement is false about double beam absorption instruments”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 25 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is false about the wavelengths of electromagnetic radiation?
Correct Answer: Option B — Energy does not depend on wavelength
Detailed Academic Explanation:
Energy does not depend on wavelength is the correct answer to “Which of the following is false about the wavelengths of electromagnetic radiation”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 26 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In Michelson Interferometer, if the reflected and transmitted beams are in phase at the beam splitter, then maximum intensity will reach the detector.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “In Michelson Interferometer, if the reflected and transmitted beams are in phase at the beam splitter, then maximum intensity will reach the detector.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 27 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the purpose of the beam splitter in double beam photometer or colorimeter?
Correct Answer: Option A — Splits beam into two equal intensity beams
Detailed Academic Explanation:
Splits beam into two equal intensity beams is the correct answer to “Which of the following is the purpose of the beam splitter in double beam photometer or colorimeter”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 28 of 40
★ PPSC
★ FPSC
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Only pyroelectric transducer or pyroelectric crystals are used as detectors in Fourier Transform Infrared Spectrophotometer (FTIR). What is the main reason for other types of thermal detectors are not being used in FTIR spectrophotometer?
Correct Answer: Option B — Slower response
Detailed Academic Explanation:
Slower response is the correct answer to “Only pyroelectric transducer or pyroelectric crystals are used as detectors in Fourier Transform Infrared Spectrophotometer (FTIR). What is the main reason for other types of thermal detectors are not being used in FTIR spectrophotometer”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 29 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
What is the unit of molar absorptivity or absorptivity which is used to determine absorbance A in Beer Lambert's formula?
Correct Answer: Option A — L mol-1 cm-1
Detailed Academic Explanation:
L mol-1 cm-1 is the correct answer to “What is the unit of molar absorptivity or absorptivity which is used to determine absorbance A in Beer Lambert's formula”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 30 of 40
★ PPSC
★ FPSC
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In Michelson's interferometer, the frequency of the detector output can be determined by translating the . . . . . . . . of movable mirror and the . . . . . . . . of monochromatic radiation.
Correct Answer: Option A — Velocity, Wavelength
Detailed Academic Explanation:
Velocity, Wavelength is the correct answer to “In Michelson's interferometer, the frequency of the detector output can be determined by translating the . . . . . . . . of movable mirror and the . . . . . . . . of monochromatic radiation.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 31 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The function of pressure regulators in the emission system of flame photometer is to have a steady flame which is free from flickers.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “The function of pressure regulators in the emission system of flame photometer is to have a steady flame which is free from flickers.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 32 of 40
★ PPSC
★ FPSC
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★ FPSC
Colorimeters are used in applications where great accuracy is required.
Correct Answer: Option B — False
Detailed Academic Explanation:
False is the correct answer to “Colorimeters are used in applications where great accuracy is required.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 33 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not an application of Flame emission photometers?
Correct Answer: Option D — Analysis of complex mixtures
Detailed Academic Explanation:
Analysis of complex mixtures is the correct answer to “Which of the following is not an application of Flame emission photometers”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 34 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Enzyme-linked immunosorbent assay (ELISA) is used for
Correct Answer: Option A — Quantifying antibody levels in blood
Detailed Academic Explanation:
Quantifying antibody levels in blood is the correct answer to “Enzyme-linked immunosorbent assay (ELISA) is used for”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 35 of 40
★ PPSC
★ FPSC
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★ FPSC
How is Tungsten Halogen lamp differs from normal Tungsten filament lamp used in absorption spectroscopy?
Correct Answer: Option B — Iodine is added to normal filling gas
Detailed Academic Explanation:
Iodine is added to normal filling gas is the correct answer to “How is Tungsten Halogen lamp differs from normal Tungsten filament lamp used in absorption spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 36 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following detectors does not require a battery and is also known as barrier layer cell?
Correct Answer: Option B — Photovoltaic cell
Detailed Academic Explanation:
Photovoltaic cell is the correct answer to “Which of the following detectors does not require a battery and is also known as barrier layer cell”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 37 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not true about Absorption spectroscopy?
Correct Answer: Option C — Reflection is kept maximum
Detailed Academic Explanation:
Reflection is kept maximum is the correct answer to “Which of the following is not true about Absorption spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 38 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with
Correct Answer: Option B — Uranium acetate
Detailed Academic Explanation:
Uranium acetate is the correct answer to “In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 39 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the principle of Golay cell which is used as a detector in IR spectroscopy?
Correct Answer: Option A — Expansion of gas upon heating
Detailed Academic Explanation:
Expansion of gas upon heating is the correct answer to “Which of the following is the principle of Golay cell which is used as a detector in IR spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 40 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Group I lists spectroscopic methods and Group II lists bimolecular applications of these methods. Match the methods in Group I with the applications in Group II . Group I Group II P. Infrared 1. Identification of functional groups Q. Circular Dichroism 2. Determination of secondary structure R. Nuclear Magnetic Resonance 3. Estimation of molecular weight 4. Determination of 3-D structure
Correct Answer: Option C — P-1, Q-2, R-4
Detailed Academic Explanation:
P-1, Q-2, R-4 is the correct answer to “Group I lists spectroscopic methods and Group II lists bimolecular applications of these methods. Match the methods in Group I with the applications in Group II . Group I Group II P. Infrared 1. Identification of functional groups Q. Circular Dichroism 2. Determination of secondary structure R. Nuclear Magnetic Resonance 3. Estimation of molecular weight 4. Determination of 3-D structure”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
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Analytical Tools In Biotechnology — Part 2
Master the next curriculum module with 40 exam-standard MCQs.
💡Examiner's Advisory for Biotechnology Engineering
In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.