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Analytical Tools In Biotechnology — Part 2 MCQs with Explanations

Practice high-yield Biotechnology Engineering MCQs on Analytical Tools In Biotechnology — Part 2 with worked academic solutions for PPSC, FPSC, NIH, NTS.

Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40

Verified Practice Questions

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Question 1 of 40
★ PPSC ★ FPSC

Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution?

Question 2 of 40
★ PPSC ★ FPSC

Which of the following has to be computed to determine transmittance and absorbance at various frequencies?

Question 3 of 40
★ PPSC ★ FPSC

Which of the following is the function of the chopper in Atomic Absorption Spectroscopy?

Question 4 of 40
★ PPSC ★ FPSC

Which of the following is not a fuel used in flame photometry?

Question 5 of 40
★ PPSC ★ FPSC

Determine the correctness or otherwise of the following Assertion (A) and the Reason (R) . Assertion: MTT assay is used to determine cell viability based on the principle of color formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the color development by converting tetrazolium soluble sail to insoluble salt.

Question 6 of 40
★ PPSC ★ FPSC

Which of the following is not a limitation of Beer Lambert's law, which gives the relation between absorption, thickness and concentration?

Question 7 of 40
★ PPSC ★ FPSC

Phototubes are more sensitive than photovoltaic cells.

Question 8 of 40
★ PPSC ★ FPSC

Velocity of electromagnetic radiation is more in a vacuum than in any medium.

Question 9 of 40
★ PPSC ★ FPSC

Which of the following is the wavelength of microwave radiation?

Question 10 of 40
★ PPSC ★ FPSC

Which of the following is not a detector used in Flame emission photometers?

Question 11 of 40
★ PPSC ★ FPSC

Which of the following is not a component of the emission system in Flame photometer?

Question 12 of 40
★ PPSC ★ FPSC

Which of the following is the advantage of prism monochromators?

Question 13 of 40
★ PPSC ★ FPSC

Electromagnetic radiation can travel through a vacuum.

Question 14 of 40
★ PPSC ★ FPSC

Which of the following is not an advantage of Laminar flow burner used in Flame photometry?

Question 15 of 40
★ PPSC ★ FPSC

The representation of Beer Lambert's law is given as A = abc. If 'b' represents distance, 'c' represents concentration and 'A' represents absorption, what does 'a' represent?

Question 16 of 40
★ PPSC ★ FPSC

Which type of Quantum Transition takes place in Ultra Violet and Visible spectroscopy?

Question 17 of 40
★ PPSC ★ FPSC

Which of the following is the function of the atomiser in the emission system of Atomic Absorption Spectroscopy?

Question 18 of 40
★ PPSC ★ FPSC

Which of the following is a source used in spectroscopy?

Question 19 of 40
★ PPSC ★ FPSC

In which of the following ways, absorption is related to transmittance?

Question 20 of 40
★ PPSC ★ FPSC

Which of the following is not a reason for laser not being generally used as a source of radiation for UV, Visible Spectroscopy?

Question 21 of 40
★ PPSC ★ FPSC

Which of the following is not a source used in Mid Infrared Spectrophotometer?

Question 22 of 40
★ PPSC ★ FPSC

In photometers, the readings of the specimen are initially obtained in the form of which of the following parameters?

Question 23 of 40
★ PPSC ★ FPSC

Beer's law states that the intensity of light decreases with respect to . . . . . . . .

Question 24 of 40
★ PPSC ★ FPSC

Protein-protein interactions are studied by P. DNA foot printing Q. Yeast two hybrid system R. Ligase chain reaction S. Mass spectrometry

Question 25 of 40
★ PPSC ★ FPSC

Which of the following technique(s) can be used to study conformational changes in myoglobin? P. Mass spectrometry Q. Fluorescence spectroscopy R. Circular dichroism spectroscopy S. Light microscopy

Question 26 of 40
★ PPSC ★ FPSC

Which of the following is the function of the Flame or Emission system in Atomic Absorption Spectroscopy?

Question 27 of 40
★ PPSC ★ FPSC

Which of the following options explains the process of 'sputtering' that occurs in Hollow Cathode Lamp?

Question 28 of 40
★ PPSC ★ FPSC

Which of the following is not a type of Spectroscopy?

Question 29 of 40
★ PPSC ★ FPSC

Which of the following is the reference that is generally used in FTIR interferometer?

Question 30 of 40
★ PPSC ★ FPSC

Which of the following is not a property or parameter of electromagnetic radiation?

Question 31 of 40
★ PPSC ★ FPSC

At what pressure should the gases in the sealed tube be maintained in the Hollow cathode lamp?

Question 32 of 40
★ PPSC ★ FPSC

Lambert's law states that the intensity of light decreases with respect to . . . . . . . .

Question 33 of 40
★ PPSC ★ FPSC

Match Group I with Group II . Group I Group II P. Real Time-PCR 1. Biochips Q. 2-D Electrophoresis 2. Syber Green R. Affinity chromatography 3. Antibody linked sephrose beads S. Microarray 4. Ampholytes

Question 34 of 40
★ PPSC ★ FPSC

Which of the following is the wavenumber of UV and Visible radiation?

Question 35 of 40
★ PPSC ★ FPSC

The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant of P that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT?

Question 36 of 40
★ PPSC ★ FPSC

Which of the following statements is false about single beam absorption instruments?

Question 37 of 40
★ PPSC ★ FPSC

In a solid sample treatment technique, the finely ground solid sample is mixed with mineral oil to make a thick paste which is then spread between IR transmitting windows. What is the name of this solid sample treatment technique?

Question 38 of 40
★ PPSC ★ FPSC

Which of the following is the purpose of balance indicator in double beam photometer or colorimeter?

Question 39 of 40
★ PPSC ★ FPSC

Beer Lambert's law gives the relation between which of the following?

Question 40 of 40
★ PPSC ★ FPSC

In Flame emission photometers, the measurement of . . . . . . . . is used for quantitative analysis.

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Analytical Tools In Biotechnology — Part 1

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💡 Examiner's Advisory for Biotechnology Engineering

In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.

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