Analytical Tools In Biotechnology — Part 2 MCQs with Explanations
Practice high-yield Biotechnology Engineering MCQs on Analytical Tools In Biotechnology — Part 2 with worked academic solutions for PPSC, FPSC, NIH, NTS.
Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40
Verified Practice Questions
Interactive test mode with detailed academic worked solutions
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Question 1 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution?
Correct Answer: Option D — NMR spectroscopy
Detailed Academic Explanation:
NMR spectroscopy is the correct answer to “Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 2 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following has to be computed to determine transmittance and absorbance at various frequencies?
Correct Answer: Option B — Ratio of sample and reference spectra
Detailed Academic Explanation:
Ratio of sample and reference spectra is the correct answer to “Which of the following has to be computed to determine transmittance and absorbance at various frequencies”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 3 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the function of the chopper in Atomic Absorption Spectroscopy?
Correct Answer: Option B — To break the steady light into a pulsating light
Detailed Academic Explanation:
To break the steady light into a pulsating light is the correct answer to “Which of the following is the function of the chopper in Atomic Absorption Spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 4 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a fuel used in flame photometry?
Correct Answer: Option D — Camphor oil
Detailed Academic Explanation:
Camphor oil is the correct answer to “Which of the following is not a fuel used in flame photometry”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 5 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Determine the correctness or otherwise of the following Assertion (A) and the Reason (R) . Assertion: MTT assay is used to determine cell viability based on the principle of color formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the color development by converting tetrazolium soluble sail to insoluble salt.
Correct Answer: Option A — Both (A) and (R) are true and (R) is the correct reason for (A)
Detailed Academic Explanation:
Both (A) and (R) are true and (R) is the correct reason for (A) is the correct answer to “Determine the correctness or otherwise of the following Assertion (A) and the Reason (R) . Assertion: MTT assay is used to determine cell viability based on the principle of color formation by DNA fragmentation. Reason: MTT assay is used to determine cell viability based on the color development by converting tetrazolium soluble sail to insoluble salt.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 6 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a limitation of Beer Lambert's law, which gives the relation between absorption, thickness and concentration?
Correct Answer: Option B — Radiation must have higher bandwidth
Detailed Academic Explanation:
Radiation must have higher bandwidth is the correct answer to “Which of the following is not a limitation of Beer Lambert's law, which gives the relation between absorption, thickness and concentration”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 7 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Phototubes are more sensitive than photovoltaic cells.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “Phototubes are more sensitive than photovoltaic cells.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 8 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Velocity of electromagnetic radiation is more in a vacuum than in any medium.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “Velocity of electromagnetic radiation is more in a vacuum than in any medium.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 9 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the wavelength of microwave radiation?
Correct Answer: Option D — 0.75 - 3.75 mm
Detailed Academic Explanation:
0.75 - 3.75 mm is the correct answer to “Which of the following is the wavelength of microwave radiation”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 10 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a detector used in Flame emission photometers?
Correct Answer: Option D — Chromatogram
Detailed Academic Explanation:
Chromatogram is the correct answer to “Which of the following is not a detector used in Flame emission photometers”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 11 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a component of the emission system in Flame photometer?
Correct Answer: Option D — Chopper
Detailed Academic Explanation:
Chopper is the correct answer to “Which of the following is not a component of the emission system in Flame photometer”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 12 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the advantage of prism monochromators?
Correct Answer: Option A — Dispersion is non-overlapping
Detailed Academic Explanation:
Dispersion is non-overlapping is the correct answer to “Which of the following is the advantage of prism monochromators”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 13 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Electromagnetic radiation can travel through a vacuum.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “Electromagnetic radiation can travel through a vacuum.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 14 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not an advantage of Laminar flow burner used in Flame photometry?
Correct Answer: Option D — Sample containing two or more solvents can be burned efficiently
Detailed Academic Explanation:
Sample containing two or more solvents can be burned efficiently is the correct answer to “Which of the following is not an advantage of Laminar flow burner used in Flame photometry”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 15 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The representation of Beer Lambert's law is given as A = abc. If 'b' represents distance, 'c' represents concentration and 'A' represents absorption, what does 'a' represent?
Correct Answer: Option C — Absorptivity
Detailed Academic Explanation:
Absorptivity is the correct answer to “The representation of Beer Lambert's law is given as A = abc. If 'b' represents distance, 'c' represents concentration and 'A' represents absorption, what does 'a' represent”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 16 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which type of Quantum Transition takes place in Ultra Violet and Visible spectroscopy?
Correct Answer: Option C — Bonding electrons
Detailed Academic Explanation:
Bonding electrons is the correct answer to “Which type of Quantum Transition takes place in Ultra Violet and Visible spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 17 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the function of the atomiser in the emission system of Atomic Absorption Spectroscopy?
Correct Answer: Option C — To break large mass of liquid into small drops
Detailed Academic Explanation:
To break large mass of liquid into small drops is the correct answer to “Which of the following is the function of the atomiser in the emission system of Atomic Absorption Spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 18 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is a source used in spectroscopy?
Correct Answer: Option D — Tungsten lamp
Detailed Academic Explanation:
Tungsten lamp is the correct answer to “Which of the following is a source used in spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 19 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In which of the following ways, absorption is related to transmittance?
Correct Answer: Option C — Absorption is the negative logarithm of transmittance
Detailed Academic Explanation:
Absorption is the negative logarithm of transmittance is the correct answer to “In which of the following ways, absorption is related to transmittance”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 20 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a reason for laser not being generally used as a source of radiation for UV, Visible Spectroscopy?
Correct Answer: Option C — Less intensity
Detailed Academic Explanation:
Less intensity is the correct answer to “Which of the following is not a reason for laser not being generally used as a source of radiation for UV, Visible Spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 21 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a source used in Mid Infrared Spectrophotometer?
Correct Answer: Option B — High pressure mercury arc lamp
Detailed Academic Explanation:
High pressure mercury arc lamp is the correct answer to “Which of the following is not a source used in Mid Infrared Spectrophotometer”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 22 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In photometers, the readings of the specimen are initially obtained in the form of which of the following parameters?
Correct Answer: Option A — Transmittance
Detailed Academic Explanation:
Transmittance is the correct answer to “In photometers, the readings of the specimen are initially obtained in the form of which of the following parameters”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 23 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Beer's law states that the intensity of light decreases with respect to . . . . . . . .
Correct Answer: Option A — Concentration
Detailed Academic Explanation:
Concentration is the correct answer to “Beer's law states that the intensity of light decreases with respect to . . . . . . . .”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 24 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Protein-protein interactions are studied by P. DNA foot printing Q. Yeast two hybrid system R. Ligase chain reaction S. Mass spectrometry
Correct Answer: Option B — Q and S only
Detailed Academic Explanation:
Q and S only is the correct answer to “Protein-protein interactions are studied by P. DNA foot printing Q. Yeast two hybrid system R. Ligase chain reaction S. Mass spectrometry”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 25 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following technique(s) can be used to study conformational changes in myoglobin? P. Mass spectrometry Q. Fluorescence spectroscopy R. Circular dichroism spectroscopy S. Light microscopy
Correct Answer: Option C — Q and R only
Detailed Academic Explanation:
Q and R only is the correct answer to “Which of the following technique(s) can be used to study conformational changes in myoglobin? P. Mass spectrometry Q. Fluorescence spectroscopy R. Circular dichroism spectroscopy S. Light microscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 26 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the function of the Flame or Emission system in Atomic Absorption Spectroscopy?
Correct Answer: Option D — To reduce the sample into atomic state
Detailed Academic Explanation:
To reduce the sample into atomic state is the correct answer to “Which of the following is the function of the Flame or Emission system in Atomic Absorption Spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 27 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following options explains the process of 'sputtering' that occurs in Hollow Cathode Lamp?
Correct Answer: Option A — Positive ions collide with cathode surface and metal atoms from cathode are ejected
Detailed Academic Explanation:
Positive ions collide with cathode surface and metal atoms from cathode are ejected is the correct answer to “Which of the following options explains the process of 'sputtering' that occurs in Hollow Cathode Lamp”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 28 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a type of Spectroscopy?
Correct Answer: Option D — Sound
Detailed Academic Explanation:
Sound is the correct answer to “Which of the following is not a type of Spectroscopy”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 29 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the reference that is generally used in FTIR interferometer?
Correct Answer: Option A — Air
Detailed Academic Explanation:
Air is the correct answer to “Which of the following is the reference that is generally used in FTIR interferometer”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 30 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a property or parameter of electromagnetic radiation?
Correct Answer: Option B — Voltage
Detailed Academic Explanation:
Voltage is the correct answer to “Which of the following is not a property or parameter of electromagnetic radiation”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 31 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
At what pressure should the gases in the sealed tube be maintained in the Hollow cathode lamp?
Correct Answer: Option A — 1 to 5 torr
Detailed Academic Explanation:
1 to 5 torr is the correct answer to “At what pressure should the gases in the sealed tube be maintained in the Hollow cathode lamp”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 32 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Lambert's law states that the intensity of light decreases with respect to . . . . . . . .
Correct Answer: Option B — Distance
Detailed Academic Explanation:
Distance is the correct answer to “Lambert's law states that the intensity of light decreases with respect to . . . . . . . .”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 33 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Match Group I with Group II . Group I Group II P. Real Time-PCR 1. Biochips Q. 2-D Electrophoresis 2. Syber Green R. Affinity chromatography 3. Antibody linked sephrose beads S. Microarray 4. Ampholytes
Correct Answer: Option C — P-2, Q-4, R-3, S-1
Detailed Academic Explanation:
P-2, Q-4, R-3, S-1 is the correct answer to “Match Group I with Group II . Group I Group II P. Real Time-PCR 1. Biochips Q. 2-D Electrophoresis 2. Syber Green R. Affinity chromatography 3. Antibody linked sephrose beads S. Microarray 4. Ampholytes”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 34 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the wavenumber of UV and Visible radiation?
Correct Answer: Option A — 1 x 106 to 1.3 x 104 m-1
Detailed Academic Explanation:
1 x 106 to 1.3 x 104 m-1 is the correct answer to “Which of the following is the wavenumber of UV and Visible radiation”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 35 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant of P that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT?
Correct Answer: Option A — P will migrate faster than Q towards the anode
Detailed Academic Explanation:
P will migrate faster than Q towards the anode is the correct answer to “The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant of P that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 36 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following statements is false about single beam absorption instruments?
Correct Answer: Option B — Beam splitter is used to get parallel beam
Detailed Academic Explanation:
Beam splitter is used to get parallel beam is the correct answer to “Which of the following statements is false about single beam absorption instruments”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 37 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In a solid sample treatment technique, the finely ground solid sample is mixed with mineral oil to make a thick paste which is then spread between IR transmitting windows. What is the name of this solid sample treatment technique?
Correct Answer: Option B — Mull technique
Detailed Academic Explanation:
Mull technique is the correct answer to “In a solid sample treatment technique, the finely ground solid sample is mixed with mineral oil to make a thick paste which is then spread between IR transmitting windows. What is the name of this solid sample treatment technique”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 38 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the purpose of balance indicator in double beam photometer or colorimeter?
Correct Answer: Option D — Indicates the difference between the output of two photometers
Detailed Academic Explanation:
Indicates the difference between the output of two photometers is the correct answer to “Which of the following is the purpose of balance indicator in double beam photometer or colorimeter”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 39 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Beer Lambert's law gives the relation between which of the following?
Correct Answer: Option C — Energy absorption and concentration
Detailed Academic Explanation:
Energy absorption and concentration is the correct answer to “Beer Lambert's law gives the relation between which of the following”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
Question 40 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In Flame emission photometers, the measurement of . . . . . . . . is used for quantitative analysis.
Correct Answer: Option B — Intensity
Detailed Academic Explanation:
Intensity is the correct answer to “In Flame emission photometers, the measurement of . . . . . . . . is used for quantitative analysis.”. This question is standard in competitive examination syllabi for Analytical Tools In Biotechnology.
💡Key Conceptual Anchor:Analytical Tools In Biotechnology
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Analytical Tools In Biotechnology — Part 1
Master the next curriculum module with 40 exam-standard MCQs.
💡Examiner's Advisory for Biotechnology Engineering
In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.