Animal Biotechnology — Part 1 MCQs with Explanations

Neomycin selects for the survival of embryonic stem cells (ES) that have incorporated the mutant gene X anywhere in the genome is the correct answer to “What is the primary purpose of neomycin in creating mice with knockouts in gene X”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Remove mycoplasma and microorganisms is the correct answer to “The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to”. This question is standard in competitive examination syllabi for Animal Biotechnology.

None of the above is the correct answer to “During the media preparation for cultivation of cells, insoluble precipitates of calcium phosphates are often formed, identify which method can be adopted to avoid this problem”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Introduce foreign DNA into a gamete or fertilized ovum is the correct answer to “To create a transgenic organism:”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Genetic engineering is the correct answer to “When scientists make an animal superior by view of genotype, introducing some foreign genes in it, the phenomenon is called”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Airlift fermenter is the correct answer to “The following culture systems are used for growing large amount of anchorage dependent animal cells except”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Insulin is the correct answer to “Which of the following promotes glucose and amino acid uptake by muscle”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Two different ori sites at different times in opposite directions is the correct answer to “Mitochondrial DNA is replicated from”. This question is standard in competitive examination syllabi for Animal Biotechnology.

I, II and III is the correct answer to “In transgenics, alteration in the sequence of nucleotide in genes are due to I. Subsitution II. Deletion II. Insertion IV. Rearrangement”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Store of calcium ions is the correct answer to “The sarcoplasmic reticulum in muscle cells acts as a”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Recombinant DNA is the correct answer to “A molecule that consists of a piece of DNA from one organism, combined with the DNA from a member of another species is called”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Liposome transfer is the correct answer to “Which gene transfer technique involves the use of a fatty bubble to carry a gene into a somatic cell”. This question is standard in competitive examination syllabi for Animal Biotechnology.

P, S is the correct answer to “Identify the correct statements for the 'HAT medium'. P. Includes drug aminopterin to block major pathway for synthesis of deoxyribonucleotides. Q. Hypoxanthine is precursor for thymidine. R. Includes drug aminopterin to block major pathway for synthesis of polypeptides. S. Cells can grow in presence of aminopterin only if they have enzymes thymidine kinase and hypoxanthine-guanine phosphoribosyl transferase.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Anaerobic energy production is the correct answer to “In general, the higher the intensity of exercise, the greater the proportional contribution of”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Two molecules of ATP is the correct answer to “The conversion of one molecule of glucose to two molecules of pyruvate results in the net formation of”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Phenol red is the correct answer to “Which one of the following is used as a pH indicator in animal cell culture medium”. This question is standard in competitive examination syllabi for Animal Biotechnology.

One gram of palmitic acid is the correct answer to “Which of the following releases most energy when completely oxidised in the body”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Trypsin/Collag is the correct answer to “Which of the following reagents is used for harvesting anchorage-dependent animal cells from culture vessels”. This question is standard in competitive examination syllabi for Animal Biotechnology.

DNA replication promotes positive supercoiling downstream from the replication fork is the correct answer to “Which of the following statements regarding the supercoiling of duplex DNA is correct”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Actin and myosin is the correct answer to “The two principal contractile proteins found in skeletal muscle are:”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Plasmid only is the correct answer to “The blue colonies contained”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Beautiful DNA is the correct answer to “Which of the following is a false term”. This question is standard in competitive examination syllabi for Animal Biotechnology.

NaHCO3 is the correct answer to “Which one of the following media components is used to maintain pH in mammalian cell culture”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Gene expression profiling is the correct answer to “Which of the following would be used to study which genes are transcribed in a particular cell line”. This question is standard in competitive examination syllabi for Animal Biotechnology.

β-lactoglobulin is the correct answer to “A heterologous protein for its expression in the milk of a transgenic animal should be under the control of the promoter of the gene coding for”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Transgenic is the correct answer to “A multicellular organism that carries a specific genetic change in each cell bacause of an intervention at the fertilized egg stage is a”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Gluconeogenesis is the correct answer to “The synthesis of glucose from lactate, glycerol, or amino acids is called”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Metastases is the correct answer to “Cells that leave a tumor and spread through out the body forming tumors at distant sites are called”. This question is standard in competitive examination syllabi for Animal Biotechnology.

50,000 - 100,000 is the correct answer to “The number of replicons in a typical mammalian cell is”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Both A and B is the correct answer to “In the above problem, colonies that were white in the presence of X-gal contained”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Both I and II are true is the correct answer to “HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine. II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Radioimmunoassay is the correct answer to “Which among the following immunological techniques is based on passive diffusion of antigen and antibody towards each other”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Breakdown of starch is the correct answer to “Alpha and Beta amylase help in”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Fertilized embryo is the correct answer to “Embryonic stem cells are derived from”. This question is standard in competitive examination syllabi for Animal Biotechnology.

It protects cells by preventing crystallization of water is the correct answer to “Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because”. This question is standard in competitive examination syllabi for Animal Biotechnology.

P, R and S is the correct answer to “Somatic cell gene transfer is used for P. Transgenic animal production. Q. Transgenic diploid cell production. R. In vitro fertilization. S. Classical breeding of farm animals.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Synthesis of purine deoxynucleotides by de novo pathway is the correct answer to “Cells deficient in hypoxanthine guanine phosphoribosyl-transferase (HPRT) enzyme rely on”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Anchorage-independent cell division is the correct answer to “An animal cell line was transected with DNA extracted from a tumorous tissue. Which one of the following will be diagnostic for its tumorous transformation”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Both A and B is the correct answer to “Which are the most commonly used enzymes for separating cells in a given tissue for culture”. This question is standard in competitive examination syllabi for Animal Biotechnology.

(i) 106 ml, (ii) 3.18 × 1016 is the correct answer to “Bacillus subtilis under ideal conditions occupies 5% of the total volume of a fermenter. Under such conditions; the culture medium (after bacilli are filtered off) has a concentration of 1mg/ml of the secreted protein trehalae (i) Calculate the volume of culture filtrate required to purify 1 kg of trehalase (ii) What are the total number of B. subtilis cells which produce this amount of enzyme? Assume that each cell is a cylinder which has dimensions of 1 m diameter and length 2μm.”. This question is standard in competitive examination syllabi for Animal Biotechnology.