Basic Principles Of Biotechnology — Part 1 MCQs with Explanations

RNase P is the correct answer to “Which of the following is a prime example of a ribonucleoprotein enzyme, the catalytic activity of which (under non-physiological conditions), resides solely in its RNA component”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

P, Q is the correct answer to “Main functions of baffles in a bioreactor are P. To prevent a vortex. Q. To increase aeration. R. To reduce interfacial area of oxygen transfer. S. To reduce aeration rate.”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Antibiotics prevent the release of energy from ATP is the correct answer to “All the following may be methods for the inhibition of microbial growth by antibiotics except”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

An alpha-helical region of about 20 to 25 hydrophobic amino acids is the correct answer to “A feature common to almost all transmembrane proteins is”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Freeze-fracture electron microscopy is the correct answer to “The distribution of transmembrane protein in the palne of a cell membrane can best be visualized by which of the following:”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

On one strand there were looped out regions of single-stranded DNA is the correct answer to “Broker, Chow, Roberts and P. Sharp hybridized mRNA from adenovirus to denatured adenovirus DNA and examined the resultant duplex molecules by electron microscopy. Which of the following did they observe”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Transcription of mRNA would not occur is the correct answer to “If a mutation occurred in the phosphorylation site in the CTD domain of RNA polymerase II, which of the following outcomes would be observed”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

10μ is the correct answer to “A microorganism when viewed under a compound microscope with objective of 40X and eye piece of 10X magnification measured 400μ in length. The same microorganism when viewed under a dissecting microscope with 10X lens, would measure”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Homologous recombination is the correct answer to “Which of the following technique is used to inactivate a gene by altering the DNA encoding it”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

During meiosis, the expected 2 : 2 ratio of alleles is replaced with a 3 : 1 ratio is the correct answer to “Gene conversion is observed”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Vernalisation is the correct answer to “The physiological mechanism responsible for flowering in response to temperature”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Asp is the correct answer to “Which of the following amino acids have side chains that are negatively charged under physiological conditions (i.e., near pH 7)”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Trypsin is the correct answer to “Histones have very high percentage of basic amino acids (15 - 30%). For this class of proteins which of the following reagents would be a suitable choice for generating peptides in determination of amino acid sequence of the protein”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Zero is the correct answer to “Number of nucleotide present in FMN is”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

0 is the correct answer to “If a reaction is at equilibrium, the free energy, ΔG change is”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

4-8-3-7-2-5-6-1 is the correct answer to “Order the following events observed during muscle contraction in the correct sequence 1. The sarcomere shortens 2. ATP is hydrolyzed 3. Ca 2+ binds to troponin 4. ATP binds to myosin 5. Mosin contracts F-actin 6. F-actin moves 7. Tropomyosin moves 8. Ca 2+ is released from the sarcoplasmic reticulum”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

O2 is the correct answer to “Under standard temperature (T) and pressure (P) conditions, 128 g of an ideal gas molecule A occupies a volume of 1 L. The gas molecule A obeys the relationship RT = 0.25 PV. R and V are universal gas constant and ideal gas volume, respectively. The molecule A is”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

-2 is the correct answer to “A peptide has the sequence Glu-His-Trp-Ser- Gly-Leu-Arg-pro-Gly. What is the net charge of the molecule at PH 11.”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Q and S is the correct answer to “Which of the following statements are correct? Very long DNA fragments (> 100 kb) from eukaryotic genomes P. Can be efficiency packaged in a λ phage vector Q. Can be propagated in yeast artificial chromosomes R. Must have cohesive ends for cloning S. Can be separated by pulsed field gel electrophoresis techniques”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

The carboxyl end of RNA Pol II contains a short sequence of only seven amino acids which is called the CTD and which may be phosphorylated is the correct answer to “Which one of the following statements about eukaryotic RNA polymerases I, II and III is false”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

$$pH = p{K_a} + log\frac{{[Salt]}}{{[Acid]}}$$ is the correct answer to “Henderson's equation can be respresented as”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Bind with troponin, changing its shape so that the actin filament is exposed is the correct answer to “The role of calcium in muscle contraction is to”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Gene inactivation provides an opportunity to identify phenotypic changes associated with the loss of the functional gene is the correct answer to “Why is the inactivation of a useful technique to determine the function of a gene”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Polyunsaturated fatty acids contain conjugated double bonds is the correct answer to “Which of the following statements is not true of fatty acids found in most mammalian cells”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

The pH at which the net charge on the molecule is zero is the correct answer to “The isoelectric point of a protein is defined as”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Momentum diffusivity to thermal diffusivity is the correct answer to “Prandtl number is the ratio of”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

40% is the correct answer to “Energy capture efficiency of the aerobic cells using glucose as a substrate is”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

The sequence to which it hybridizes must be complementary to that of the probe is the correct answer to “For a nucleic acid probe to hybridize, which of the following must be true”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Protein is a homodimer is the correct answer to “A homogeneous protein of native molecular weight 100,000 gave a single band of molecular weight 50,000 on SDS-PAGE in presence of β-mercaptoethanol. N-terminal analysis gave two amino acids alanine and leucine in equal proportions. Hence the”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Double-stranded RNA into short strands is the correct answer to “In the RNAi regulatory pathway, the DICER enzyme cleaves”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

W m-2 K-1 is the correct answer to “Which one of the following is the unit of heat transfer coefficient”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

All of these is the correct answer to “Amplified fragment length polymorphism (AFLP) P. A PCR-based tool Q. Uses restriction enzymes to cut genomic DNA R. Involves ligation of adaptors to the sticky ends of the restriction fragments S. Involves amplification using primers complementary to the adaptor”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

P, S is the correct answer to “Which of the following statements are true in case of fluid-mosaic model for cell membrances P. Between 5 - 8 nm thick and appear trilaminar when viewed in cross-section under electron microscope Q. Less than 1 nm thick and consist of a layer of protein sandwitched between two layers of phospholipids R. In the lipid bilayer, proteins are embedded at irregular intervals and held by hydrophilic interactions between lipids and hydrophilic domains of the proteins S. The protein domains exposed on one side of the lipid bilayer are different from those exposed on the other side”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Helical ribbon is the correct answer to “Which one of the following is the most suitable type of impeller for mixing high viscosity (viscosity > 10 5 cP) fluids”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

In vitro, myosin assembles spontaneously into the thin filaments is the correct answer to “Which of the following statements about myosin is not true”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

They account for approximately one-fifth of the mass of a chromosome is the correct answer to “Which of the following statements about histones is incorrect”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Microsatellites are present throughout eukaryotic genomes and are easily amplified using PCR is the correct answer to “Microsatellites are used more commonly than minisatellite as DNA markers because”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Methotrexate (antifolate) is the correct answer to “Which of the following Chemotherapeutic agents works by impairing de novo synthesis”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

Allows flexibility in the choice of restriction enzymes for cloning is the correct answer to “A multiple cloning site”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.

All of the above is the correct answer to “X-ray crystallography are P. Based on the elastic scattering of X-rays Q. Non-destructive analytical techniques R. Used in determining the arrangement of atoms within a crystal S. Based on Bragg's law”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.