Basic Principles Of Biotechnology — Part 2 MCQs with Explanations
Practice high-yield Biotechnology Engineering MCQs on Basic Principles Of Biotechnology — Part 2 with worked academic solutions for PPSC, FPSC, NIH, NTS.
Questions
17
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
21 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
17
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Question 1 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Phase contrast microscope takes advantage of
Correct Answer: Option A — Density of different parts of a cell
Detailed Academic Explanation:
Density of different parts of a cell is the correct answer to “Phase contrast microscope takes advantage of”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 2 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Measurement of K L a in a bioreactor can be carried out by sodium sulfite oxidation method, that is, based on the oxidation of sodium sulfite to sodium sulfate in the presence of a catalyst (Cu ++ or Co ++ ). In a typical experiment, a laboratory fermenter was filled with 5 liter of 0.5 M sodium sulfite solution containing 0.003 M Cu ++ ions and the air was sparged in. After 10 min, the air flow was stopped and a 10 mL sample was taken and titrated. The concentration of sodium sulfite in the sample was found to be 0.20 M. The oxygen uptake rate for this aerated system will work out to be
Correct Answer: Option B — 0.008 g L-1 s-1
Detailed Academic Explanation:
0.008 g L-1 s-1 is the correct answer to “Measurement of K L a in a bioreactor can be carried out by sodium sulfite oxidation method, that is, based on the oxidation of sodium sulfite to sodium sulfate in the presence of a catalyst (Cu ++ or Co ++ ). In a typical experiment, a laboratory fermenter was filled with 5 liter of 0.5 M sodium sulfite solution containing 0.003 M Cu ++ ions and the air was sparged in. After 10 min, the air flow was stopped and a 10 mL sample was taken and titrated. The concentration of sodium sulfite in the sample was found to be 0.20 M. The oxygen uptake rate for this aerated system will work out to be”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 3 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In a well aerated and agitated microbial culture, the 'supply' of oxygen is equal to 'demand' (uptake) of the growing culture. The K L a for such a system will be (K L a = volumetric mass transfer coefficient, C* = dissolved oxygen concentration in liquid in equilibrium with gaseous oxygen, C = instantaneous value of dissolved oxygen concentration, 'r' = specific oxygen uptake rate per unit weight of cells, X = dry weight of the cells per unit volume)
$$\frac{{\left( {rX} \right)}}{{\left( {C* - C} \right)}}$$ is the correct answer to “In a well aerated and agitated microbial culture, the 'supply' of oxygen is equal to 'demand' (uptake) of the growing culture. The K L a for such a system will be (K L a = volumetric mass transfer coefficient, C* = dissolved oxygen concentration in liquid in equilibrium with gaseous oxygen, C = instantaneous value of dissolved oxygen concentration, 'r' = specific oxygen uptake rate per unit weight of cells, X = dry weight of the cells per unit volume)”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 4 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Glycogen is a branched polymer of glucose It has
Correct Answer: Option C — One reducing end and several non-reducing ends
Detailed Academic Explanation:
One reducing end and several non-reducing ends is the correct answer to “Glycogen is a branched polymer of glucose It has”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 5 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Shear stress versus shear rate behavior of four different types of fluids (I, II, III and IV) are shown in the figure below. Which one of the following options is correct?
Correct Answer: Option D — I-Newtonian, II-Bingham plastic, III-Pseudoplastic, IV-Dilatant
Detailed Academic Explanation:
I-Newtonian, II-Bingham plastic, III-Pseudoplastic, IV-Dilatant is the correct answer to “Shear stress versus shear rate behavior of four different types of fluids (I, II, III and IV) are shown in the figure below. Which one of the following options is correct”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 6 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
If the frequency of males affected with an X-linked recessive condition in a human population is 0.10 (one in ten), what will be the expected frequency of affected females?
Correct Answer: Option D — 0.01
Detailed Academic Explanation:
0.01 is the correct answer to “If the frequency of males affected with an X-linked recessive condition in a human population is 0.10 (one in ten), what will be the expected frequency of affected females”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 7 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Power number also called Newton's number, is defined as a dimensionless parameter relating to
Correct Answer: Option C — Energy required by the stirred reactors
Detailed Academic Explanation:
Energy required by the stirred reactors is the correct answer to “Power number also called Newton's number, is defined as a dimensionless parameter relating to”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 8 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In the meiosis type cell division, chromatids segregates during
Correct Answer: Option D — Anaphase II
Detailed Academic Explanation:
Anaphase II is the correct answer to “In the meiosis type cell division, chromatids segregates during”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 9 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Binding of mannose 6-Phosphate to protein lead to its localization to
Correct Answer: Option A — Lsosome
Detailed Academic Explanation:
Lsosome is the correct answer to “Binding of mannose 6-Phosphate to protein lead to its localization to”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 10 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In mammalian cells, RNA editing P. Can change the coding capacity of a gene after transcription Q. Creates a stop codon in the middle of the apo-B mRNA in intestinal cells but not in liver cells R. Usually makes many changes in each mRNA S. Can change specific nucleotides by deamination
Correct Answer: Option C — P, Q, S
Detailed Academic Explanation:
P, Q, S is the correct answer to “In mammalian cells, RNA editing P. Can change the coding capacity of a gene after transcription Q. Creates a stop codon in the middle of the apo-B mRNA in intestinal cells but not in liver cells R. Usually makes many changes in each mRNA S. Can change specific nucleotides by deamination”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 11 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
All histones undergo post-translational modification of specific amino acids. These modifications P. Alter the charge of the histone molecule Q. Are permanent R. Occur on the N-terminal tail of the histones, which are thought to extend out from the core S. Occur at specific times during the cell cycle
Correct Answer: Option C — P, R, S
Detailed Academic Explanation:
P, R, S is the correct answer to “All histones undergo post-translational modification of specific amino acids. These modifications P. Alter the charge of the histone molecule Q. Are permanent R. Occur on the N-terminal tail of the histones, which are thought to extend out from the core S. Occur at specific times during the cell cycle”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 12 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The concept of random fluctuations gene frequency as an evolutionary force was originally proposed by
Correct Answer: Option C — Sewall Wright
Detailed Academic Explanation:
Sewall Wright is the correct answer to “The concept of random fluctuations gene frequency as an evolutionary force was originally proposed by”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 13 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In a bioreactor baffles are incorporated to
Correct Answer: Option A — Prevent vortex and to improve aeration efficiency
Detailed Academic Explanation:
Prevent vortex and to improve aeration efficiency is the correct answer to “In a bioreactor baffles are incorporated to”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 14 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Cytochrome P450 family of enzymes causes detoxification mainly by
Correct Answer: Option B — Catalyzing the addition of -OH group to metabolites
Detailed Academic Explanation:
Catalyzing the addition of -OH group to metabolites is the correct answer to “Cytochrome P450 family of enzymes causes detoxification mainly by”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 15 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Telomerase activity was monitored in the following cell types. The highest amount of telomerase activity was found in the combination of
Correct Answer: Option A — Embryonic and hematopoitic stem cells
Detailed Academic Explanation:
Embryonic and hematopoitic stem cells is the correct answer to “Telomerase activity was monitored in the following cell types. The highest amount of telomerase activity was found in the combination of”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 16 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
A protein which spans that lipid bilayer
Correct Answer: Option D — Usually has both hydrophobic and hydrophilic regions
Detailed Academic Explanation:
Usually has both hydrophobic and hydrophilic regions is the correct answer to “A protein which spans that lipid bilayer”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
Question 17 of 17
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following equation represent the reciprocal of Michaelis-Menten equation
Correct Answer: Option A — $$\frac{1}{v} = \frac{{{K_m} + \left[ S \right]}}{{{V_{\max }}\left[ S \right]}}$$
Detailed Academic Explanation:
$$\frac{1}{v} = \frac{{{K_m} + \left[ S \right]}}{{{V_{\max }}\left[ S \right]}}$$ is the correct answer to “Which of the following equation represent the reciprocal of Michaelis-Menten equation”. This question is standard in competitive examination syllabi for Basic Principles Of Biotechnology.
💡Key Conceptual Anchor:Basic Principles Of Biotechnology
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Analytical Tools In Biotechnology — Part 1
Master the next curriculum module with 40 exam-standard MCQs.
💡Examiner's Advisory for Biotechnology Engineering
In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.