Animal Biotechnology — Part 2 MCQs with Explanations

All of the above is the correct answer to “In animal cell cultures, the addition of serum to media is essential for providing”. This question is standard in competitive examination syllabi for Animal Biotechnology.

An activated human gene is the correct answer to “A knockout mouse is so named because it contains”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2 is the correct answer to “In animal cell culture, a CO 2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Ti-plasrnid is the correct answer to “Which of these would not Be-used to introduce DNA in animal cells”. This question is standard in competitive examination syllabi for Animal Biotechnology.

A mouse that manufactures human protein in its milk is the correct answer to “The first patent for a transgenic organism was awarded in 1988. Which organism was patented”. This question is standard in competitive examination syllabi for Animal Biotechnology.

HbCO2 is the correct answer to “Carbon-dioxide when combined with the amino groups in haemoglobin molecule and forms”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Somatic hybridisation is the correct answer to “Term hybridoma implies”. This question is standard in competitive examination syllabi for Animal Biotechnology.

(NAD+) as an electron acceptor is the correct answer to “DNA ligase of E. coli requires which of the following cofactors”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Turner syndrome is the correct answer to “The syndrom es in human in which an individual's somatic cells contain only the one sex chromosome XO is called”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Osmotic stabilizer → Digestion of cell wall → Protoplast viability → Cybrid formation is the correct answer to “Choose the correct sequence of steps involved in cytoplast production.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Hybridoma cells are made to produce polyclonal antibodies is the correct answer to “Which one of the following statements is incorrect about hybridoma production”. This question is standard in competitive examination syllabi for Animal Biotechnology.

B cell with a myeloma cell is the correct answer to “Antibody-producing hybridoma cells are generated by the fusion of a”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Plasminogen activator is the correct answer to “Which of the following has been produced commercially from mammalian cell cultures”. This question is standard in competitive examination syllabi for Animal Biotechnology.

No survival is the correct answer to “When the value of selection coefficient is unity (S = 1), there would be”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Collagen is the correct answer to “To promote attachment and spreading of anchorage-dependent animal cells the surface of the culture vessel needs to be coated with”. This question is standard in competitive examination syllabi for Animal Biotechnology.

P, Q is the correct answer to “The culture fluids of 1000 to 5000 colonies of hybridoma are screened for monoclonal antibody by P. Western blot analysis. Q. Antigen capture analysis. R. Northern blot analysis. S. Antibody capture analysis. Choose the correct pair from the following”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Nif' genes is the correct answer to “Nitrogen fixing genes are called”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Cows; Milk is the correct answer to “Transgenic pharming involves using . . . . . . . . to produce recombinant proteins in . . . . . . . .”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Oncogenes is the correct answer to “The genes which promote cancerous growth are called as”. This question is standard in competitive examination syllabi for Animal Biotechnology.

It is an enzyme of endogeneous pathway is the correct answer to “Which of the following statement is not true for Xanthine-guanine phosphoribosyl transferase (XGPRT)”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Splenocytes is the correct answer to “Production of monoclonal antibodies by hybridoma technology requires”. This question is standard in competitive examination syllabi for Animal Biotechnology.

P-T; Q-T; R-F and S-F is the correct answer to “Choose the correct combination of True (T) and False (F) statements about microcarriers used in animal cell culture. P. Higher cell densities can be achieved using microcarriers. Q. Microcarriers increase the surface area for cell growth. R. Microcarriers are used for both anchorage- and non-anchorage-dependent cells. S. Absence of surface charge on microcarriers enhances attachment of cells.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

DNA Pol δ is the correct answer to “Which of the following DNA Pol has proofreading property in animal cells”. This question is standard in competitive examination syllabi for Animal Biotechnology.

P, Q is the correct answer to “When electroporation is used for introducing DNA into mammalian cell P. A carrier for DNA is not required. Q. The lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites. R. The viability of the cell becomes approximately zero percent. S. The first step involves absorption of DNA on the cell membrane.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

On the location of the gene in the genome is the correct answer to “Positional cloning approach exploits information”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Both [A] and [R] are true, and [R] is the correct reason for [A] is the correct answer to “Determine the correctness or otherwise of the following Assertion [A] and the Reason [R] . Assertion [A]: Embryonic stem cells are suitable for developing knockout mice. Reason [R]: Homologous recombination is more frequent in embryonic stem cells than that in somatic cells.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Early embryonic cell is the correct answer to “Gene targeting is done on an”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Chorionic gonadotropin is the correct answer to “Detection of which hormone is the commonly used test for pregnancy in humans”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Changed DHFR with reduced affinity for MTX is the correct answer to “Methotrexate (MTX) resistant cell lines developed through selection show”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Monoclonal antibodies is the correct answer to “Hybridoma technology is used to produce”. This question is standard in competitive examination syllabi for Animal Biotechnology.

A-2, B-1, C-3, D-4 is the correct answer to “Match the following reporter gene with their source Column-I Column-II A. β-Galactosidase 1. Firefly B. Lux 2. E. coli C. TK 3. Herpers simplex virus D. Blasticidin deaminase (bed) 4. Aspergillus terrcus”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Interleukin-2 is the correct answer to “For the growth of T-cell, the growth factor needed would be”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Thymidine, Hypoxanthine, Aminopterin is the correct answer to “A hybrid derived from the fusion of a myeloma cell (HPRT − ) with an antibody secreting B-lymphocyte (HPRT + ) can be selected to produce monoclonal antibody by growing in a medium containing”. This question is standard in competitive examination syllabi for Animal Biotechnology.

0.15 hr is the correct answer to “A bacterial culture contains 108 cells/ml in the beginning. Two hours later, it was found to contain 10 12 cells/ml. Calculate the doubling time.”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Option "Recombinant Erythropoietin" is the correct answer for this question under Animal Biotechnology.

105 is the correct answer to “Receptor R is over expressed in CHO cells and analyzed for expression. 6 × 10 7 cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm. the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Coulter counter is the correct answer to “Which one of the following is NOT used for the measurement of cell viability in animal cell culture”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Germline cells is the correct answer to “Gene therapy that is hereditary relates to”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Phospholipase A is the correct answer to “All of the following are produced by animal cells in culture and help the cells adhere to the culture dish except”. This question is standard in competitive examination syllabi for Animal Biotechnology.

Tissue plasminogen activator is the correct answer to “The product commercially produced by animal cell culture is”. This question is standard in competitive examination syllabi for Animal Biotechnology.