Biological Tools And Techniques — Part 1 MCQs with Explanations
Practice high-yield Biotechnology Engineering MCQs on Biological Tools And Techniques — Part 1 with worked academic solutions for PPSC, FPSC, NIH, NTS.
Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40
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Question 1 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Sodium and potassium in rock and soil materials are generally estimated by
Correct Answer: Option B — Flame photometry
Detailed Academic Explanation:
The correct answer is B: Flame photometry * Flame photometry is a technique specifically designed to measure the concentration of certain elements, like sodium (Na) and potassium (K) , based on their emission of light when introduced into a flame. * Here's why the other options are less suitable: * UV-Visible spectrophotometry: This technique measures how much UV or visible light a substance absorbs. It's good for identifying and quantifying organic molecules, but not ideal for directly measuring sodium or potassium in a complex matrix like rock or soil. * HPLC (High-Performance Liquid Chromatography): This is a separation technique. It separates different components in a liquid mixture. While it can be coupled with detectors that could measure sodium and potassium , it's not the primary or most efficient method for just measuring those elements. * Colorimetry: This method measures the absorbance of light by a colored solution to determine the concentration of a substance. While some reactions can be developed that produce colored compounds from sodium or potassium, it's generally more complex and less accurate than flame photometry for direct measurement of these elements. So, because Flame photometry directly measures the light emitted by sodium and potassium when heated in a flame, it's the most appropriate technique for estimating their concentration in rock and soil samples.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 2 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which concentration of ethanol (ethyl alcohol) is the most effective as a disinfectant?
Correct Answer: Option B — 70%
Detailed Academic Explanation:
70% is the correct answer to “Which concentration of ethanol (ethyl alcohol) is the most effective as a disinfectant”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 3 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following method works primarily by denaturing proteins?
Correct Answer: Option B — Heat
Detailed Academic Explanation:
Heat is the correct answer to “Which of the following method works primarily by denaturing proteins”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 4 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Dioxins and furans are two toxic gaseous pollutants which can be measured by
Correct Answer: Option A — GC - MS
Detailed Academic Explanation:
GC - MS is the correct answer to “Dioxins and furans are two toxic gaseous pollutants which can be measured by”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 5 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
HEPA filters are used to filter
Correct Answer: Option A — Air
Detailed Academic Explanation:
Air is the correct answer to “HEPA filters are used to filter”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 6 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
What is the relationship between resolving power of a microscope and wavelength?
Correct Answer: Option A — Shorter the wavelength, Higher the resolving power
Detailed Academic Explanation:
Shorter the wavelength, Higher the resolving power is the correct answer to “What is the relationship between resolving power of a microscope and wavelength”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 7 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following techniques is most suitable for the estimation of organic or their residues?
Correct Answer: Option C — Gas chromatography
Detailed Academic Explanation:
Gas chromatography is the correct answer to “Which of the following techniques is most suitable for the estimation of organic or their residues”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 8 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In which type of chromatography four modes viz., absorption, partition, ion exchange and exclusion, are present?
Correct Answer: Option A — HPLC
Detailed Academic Explanation:
HPLC is the correct answer to “In which type of chromatography four modes viz., absorption, partition, ion exchange and exclusion, are present”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 9 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a molecular method for detection of microbial population from waterl soil?
Correct Answer: Option B — MPN
Detailed Academic Explanation:
MPN is the correct answer to “Which of the following is not a molecular method for detection of microbial population from waterl soil”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 10 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
An ultraviolet light source is used in
Correct Answer: Option D — Fluorescent microscope
Detailed Academic Explanation:
Fluorescent microscope is the correct answer to “An ultraviolet light source is used in”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 11 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Element contaminating the water body is determined and quantified by using one of the following methods
Correct Answer: Option D — All the above
Detailed Academic Explanation:
All the above is the correct answer to “Element contaminating the water body is determined and quantified by using one of the following methods”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 12 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following technique does not give a viable count?
Correct Answer: Option D — Dry weight determination
Detailed Academic Explanation:
Dry weight determination is the correct answer to “Which of the following technique does not give a viable count”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 13 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The most effective germicidal wavelength of UV is
Correct Answer: Option B — 260 nm
Detailed Academic Explanation:
260 nm is the correct answer to “The most effective germicidal wavelength of UV is”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 14 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which one of the following is used to study the structural details of biological tissues using freezefracture technique?
Correct Answer: Option B — Transmission electron microscopy
Detailed Academic Explanation:
Transmission electron microscopy is the correct answer to “Which one of the following is used to study the structural details of biological tissues using freezefracture technique”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 15 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following instruments is based on Bragg's law?
Correct Answer: Option B — XRD
Detailed Academic Explanation:
XRD is the correct answer to “Which of the following instruments is based on Bragg's law”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 16 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Sedimentation efficiency depends on the relative strength of
Correct Answer: Option B — Drag versus gravitational force
Detailed Academic Explanation:
Drag versus gravitational force is the correct answer to “Sedimentation efficiency depends on the relative strength of”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 17 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The typical temperature for autoclaving (operating at 15 pounds per square inch of pressure) is
Correct Answer: Option A — 121°C
Detailed Academic Explanation:
121°C is the correct answer to “The typical temperature for autoclaving (operating at 15 pounds per square inch of pressure) is”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 18 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
To understand the 3D structure of the molecule following technique is called
Correct Answer: Option A — X - ray diffraction
Detailed Academic Explanation:
X - ray diffraction is the correct answer to “To understand the 3D structure of the molecule following technique is called”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 19 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
When certain types of molecules absorb UV energy, energy in the UV or visible regions is emitted as the excess energy. This phenomenon is known as
Correct Answer: Option A — Fluorescence
Detailed Academic Explanation:
Fluorescence is the correct answer to “When certain types of molecules absorb UV energy, energy in the UV or visible regions is emitted as the excess energy. This phenomenon is known as”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 20 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The band labelled as UV - C which is lethal to microorganisms is
Correct Answer: Option B — 0.2 - 0.29 μm
Detailed Academic Explanation:
0.2 - 0.29 μm is the correct answer to “The band labelled as UV - C which is lethal to microorganisms is”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 21 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is not a chemical agent causing mutation?
Correct Answer: Option B — UV light
Detailed Academic Explanation:
UV light is the correct answer to “Which of the following is not a chemical agent causing mutation”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 22 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following instrument is most suitable to estimate heavy metals from the effluent?
Correct Answer: Option B — Atomic absorption spectrophotometer
Detailed Academic Explanation:
Atomic absorption spectrophotometer is the correct answer to “Which of the following instrument is most suitable to estimate heavy metals from the effluent”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 23 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
One of the following platforms is used for whole cell fingerprinting?
Correct Answer: Option D — MALDI-TOF
Detailed Academic Explanation:
MALDI-TOF is the correct answer to “One of the following platforms is used for whole cell fingerprinting”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 24 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of these required quantum nature of light for their explanation?
Correct Answer: Option A — Diffraction
Detailed Academic Explanation:
Diffraction is the correct answer to “Which of these required quantum nature of light for their explanation”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 25 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
CO can be suitably determined by
Correct Answer: Option B — Potentiometry
Detailed Academic Explanation:
Potentiometry is the correct answer to “CO can be suitably determined by”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 26 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which one of the following techniques can be used for determining the level of cadmium in soil?
Correct Answer: Option B — Atomic absorption spectrophotometer
Detailed Academic Explanation:
Atomic absorption spectrophotometer is the correct answer to “Which one of the following techniques can be used for determining the level of cadmium in soil”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 27 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Sodium is usually estimated by which of the following analytical technique
Correct Answer: Option A — Flame photometry
Detailed Academic Explanation:
Flame photometry is the correct answer to “Sodium is usually estimated by which of the following analytical technique”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 28 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Beer - Lambert's law defines
Correct Answer: Option A — The degree of absorption of monochromatic light by a homogenous medium
Detailed Academic Explanation:
The degree of absorption of monochromatic light by a homogenous medium is the correct answer to “Beer - Lambert's law defines”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 29 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which one of the following is the best tool to study the interacting residues in protein - ligand interaction?
Correct Answer: Option A — X - ray crystallography
Detailed Academic Explanation:
X - ray crystallography is the correct answer to “Which one of the following is the best tool to study the interacting residues in protein - ligand interaction”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 30 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Hollow cathode lamp is used in
Correct Answer: Option C — Atomic absorption spectrophotometer
Detailed Academic Explanation:
Atomic absorption spectrophotometer is the correct answer to “Hollow cathode lamp is used in”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 31 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which is used to separate molecules on the basis of their sizes?
Correct Answer: Option B — Molecular exclusion chromatography
Detailed Academic Explanation:
Molecular exclusion chromatography is the correct answer to “Which is used to separate molecules on the basis of their sizes”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 32 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The heat sensitive components of microbial medium are sterilized by
Correct Answer: Option D — Membrane filtration
Detailed Academic Explanation:
Membrane filtration is the correct answer to “The heat sensitive components of microbial medium are sterilized by”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 33 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The instrument used for determination of transparency of water is
Correct Answer: Option C — Conductivity bridge
Detailed Academic Explanation:
Conductivity bridge is the correct answer to “The instrument used for determination of transparency of water is”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 34 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Remote sensing of ocean-atmospheric parameters carried out in the microwave channels is based on the phenomenon of
Correct Answer: Option A — Emission
Detailed Academic Explanation:
Emission is the correct answer to “Remote sensing of ocean-atmospheric parameters carried out in the microwave channels is based on the phenomenon of”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 35 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
If one wishes to measure total As, As +3 and As +5 in a sample the most suitable analytical tool is
Correct Answer: Option A — Atomic absorption spectrophotometer
Detailed Academic Explanation:
Atomic absorption spectrophotometer is the correct answer to “If one wishes to measure total As, As +3 and As +5 in a sample the most suitable analytical tool is”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 36 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Snow blindness is caused due to
Correct Answer: Option B — Excessive flux of visible radiations
Detailed Academic Explanation:
Excessive flux of visible radiations is the correct answer to “Snow blindness is caused due to”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 37 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following experimental methods is not used to determine three-dimensional structures of biological macromolecules?
Correct Answer: Option B — Fluorescence spectroscopy
Detailed Academic Explanation:
Fluorescence spectroscopy is the correct answer to “Which of the following experimental methods is not used to determine three-dimensional structures of biological macromolecules”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 38 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following instrument cap map the concentration of SO 2 over an area of land?
Correct Answer: Option A — LIDAR
Detailed Academic Explanation:
LIDAR is the correct answer to “Which of the following instrument cap map the concentration of SO 2 over an area of land”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 39 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following treatment achieves sterilization?
Correct Answer: Option A — Autoclaving
Detailed Academic Explanation:
Autoclaving is the correct answer to “Which of the following treatment achieves sterilization”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
Question 40 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following would be the fastest way to kill endospores?
Correct Answer: Option A — Autoclave at 121°C
Detailed Academic Explanation:
Autoclave at 121°C is the correct answer to “Which of the following would be the fastest way to kill endospores”. This question is standard in competitive examination syllabi for Biological Tools And Techniques.
💡Key Conceptual Anchor:Biological Tools And Techniques
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Analytical Tools In Biotechnology — Part 1
Master the next curriculum module with 40 exam-standard MCQs.
💡Examiner's Advisory for Biotechnology Engineering
In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.