Bioprocess Engineering And Process Biotechnology — Part 1 MCQs with Explanations

Covalent linkage to a spacer arm is the correct answer to “Which of the following techniques is best suited for immobilizing an affinity ligand”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Intracellular carbon source is the correct answer to “Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-3, Q-4, R-2, S-1 is the correct answer to “During cultivation of microorganisms in a fermenter, various parameters are controlled by appropriate sensor (probe). Match each probe in Group I with the appropriate response mechanism in Group II . Group I (Probe) Group II (Response) P. Thermistor 1. Activation of acid/alkali pump Q. Oxygen electrode 2. Activation of vegetable oil pump R. Metal rod 3. Activation of hot/cold water pump S. pH electrode 4. Increase/decrease in stirrer motor speed”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

4.6 h is the correct answer to “The kinetics of the disintegration of baker's yeast cells in a bead mill is described as $$\frac{{dp}}{{dt}} = K\left( {{P_m} - P} \right)$$ , where P is the concentration of protein released, m is the maximum protein concentration achievable, K is the first order rate constant and is 0.5 h −1 for the system studied. The time required for the release of 90% of the intracellular proteins will be:”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

R, Q, P is the correct answer to “A mixture contains three similarly sized peptides P, Q and R. The peptide P is positively charged, Q is weakly negative, and R is strongly negative. If this mixture is passed through an ion-exchange chromatography column containing an anionic resin, their order of elution will be”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-2, Q-1, R-4, S-3 is the correct answer to “Match the entries in Group I with the methods of sterilization in Group II . Group I Group II P. Serum 1. Autoclave Q. Luria broth 2. Membrane filtration R. Polypropylene tubes 3. UV irradiation S. Biological safety cabinets 4. Gamma irradiation 5. Dry heat”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-3, Q-2, R-1, S-4 is the correct answer to “Match the microbial growth characteristics in Group I with the corresponding features in Group II . Group I Group II P. Growth associated product formation 1. Specific growth rate decreases with increasing product concentration Q. Non-growth associated product formation 2. Specific product formation rate is constant R. Product inhibition 3. Specific product formation rate is proportional to specific growth rate S. Substrate inhibition 4. Specific growth rate decreases with increasing substrate concentration”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

\[0.98\,{h^{ - 1}}\] is the correct answer to “In a chemostat, evaluate the dilution rate at the cell washout condition by applying Monod's model with the given set of data: \[{\mu _{\max }} = 1\,{h^{ - 1}};{Y_{\frac{X}{S}}} = 0.5\,g\,{g^{ - 1}};{K_S} = 0.2\,g\,{L^{ - 1}};{S_0} = 10\,g\,{L^{ - 1}}\]”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Growth of revertants is the correct answer to “Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was gradually decreasing. Microbiological assays of reactor samples showed absence of contamination and recorded data showed no change in the operating conditions. The most probable reason for decrease in lysine concentration may be attributed to”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

52 is the correct answer to “In a fed-batch culture, 200 g L -1 glucose solution is added at a flow rate of 50 L h -1 . The initial culture volume (at quasi steady state) and the initial cell concentration are 600 L and 20 g L -1 , respectively. The yield coefficient \[\left( {{Y_{\frac{X}{S}}}} \right)\] is 0.5 g cell mass-g substrate -1 . The cell concentration (g L -1 ) at quasi steady state at t = 8 h is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

All of the above is the correct answer to “The following crosslinking agents may be used for the immobilization of enzymes.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

110 min is the correct answer to “Thermal death of microorganisms in the liquid medium follows first order kinetics. If the initial cell concentration in the fermentation medium is 10 8 cells mL -1 and the final acceptable contamination level is 10 -3 cells, for how long should 1 m 3 medium be treated at temperature of 120°C (thermal deactivation rate constant = 0.23 min -1 ) to achieve acceptable load”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-1, Q-2, R-4, S-3 is the correct answer to “Match separation methods in Group I with associated properties in Group II . Group I Group II P. Centrifugation 1. Density Q. Dialysis 2. Diffusivity R. Solvent extraction 3. Size S. Ultrafiltration 4. Solubility”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Inversely proportional to the viscosity of the solution is the correct answer to “In dead-end filtration, rate of filtration is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

The initial corrective action is greater for larger error is the correct answer to “Which one of the following statements is correct about proportional controllers”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Log phase is the correct answer to “A batch culture fermentation was being conducted with Streptomyces rimosus, Analysis of samples collected indicated doubling of cell number per unit time. Your interference would be that the culture is in the”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

0.50 is the correct answer to “In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 g L -1 (DCW), the specific growth rate (μ) is 0.4 h -1 and substrate uptake rate (v) is 16 g L -1 h -1 . The cell yield coefficient \[{Y_{\frac{X}{S}}}\] will be”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Diffusion is the correct answer to “The separation principle of dialysis used in the recovery of fermentation products is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

.4 × 10-4 M is the correct answer to “Evaluate the Michaelis constant for the following lipase catalyzed transesterification reaction for the production of biodiesel. $${\text{Vegetale oil}} + {\text{Lipase}} + \mathop \rightleftharpoons \limits_{{{\text{k}}1}}^{{{\text{k}}{ - 1}}} {\text{ Oil-lipase complex }}\mathop \to \limits^{{{\text{k}}_1}} {\text{Biodiesel}} + {\text{Glycerol}}$$ where k 1 = 3 × 10 8 M −1 s −1 ; k −1 = 4 × 10 4 s −1 and k 2 = 2 × 10 3 s −1”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Immobilization is the correct answer to “Which of the following is employed for the repeated use of enzymes in bioprocesses”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

It uses longer heating time during which heat is lost is the correct answer to “Choose the correct completion of the following statement. A fermenter sterilization in situ is less efficient than continuous heat sterilization because”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

All of the above is the correct answer to “Mechanism of separation of contaminants present in air by fibrous media are”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-4, Q-3, R-1, S-2 is the correct answer to “Match the entries in the Group I with the elution conditions in Group II . Group I Group II P. Ion exchange chromatography 1. Isocratic solvent Q. Hydrophobic column chromatography 2. Ampholytes R. Gel filtration chromatography 3. Increasing gradient of salt S. Chromatofocusing 4. Decreasing gradient of polarity”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

A positively charged substrate and a negatively charged product is the correct answer to “Which of the following cases are likely to lead to faster rates of catalysis by an enzyme immobilized on a negatively charged support”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Inhibition of glucose utilization and lactate accumulation in glycolysis is the correct answer to “The Pasteur Effect is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P and R only is the correct answer to “Fed batch cultivation is suitable for which of the following? P. Processes with substrate inhibition Q. Processes with product inhibition R. High cell density cultivation”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Harvesting of cells is the correct answer to “Ultrafiltration process cannot be used for”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Volumetric mass transfer coefficient is the correct answer to “For scaling up for a bioreactor, the following parameter is assumed to be constant”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P, Q and S only is the correct answer to “Which of the following events occur during the stationary phase of bacterial growth? P. Rise in cell number stops. Q. Spore formation in some Gram-positive bacteria such as Bacillus subtilis. R. Cell size increases in some Gram-negative bacteria such as Escherichia coli. S. Growth rate of bacterial cells nearly equals their death rate. T. Decrease in peptidoglycan cross-linking.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Stationary phase is the correct answer to “In a batch culture of Penicillium chrysogenum, the maximum penicillin synthesis occurs during the”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

0.65 is the correct answer to “Biomass is being produced in a continuous stirred tank bioreactor of 750 L capacity. The sterile feed containing 8 g L -1 glucose as substrate was fed at a flow rate of 150 L h -1 . The microbial system follows Monod's model with μ m = 0.4 h -1 , K S = 1.5 g L -1 and \[{Y_{\frac{X}{S}}}\] = 0.5 g cell mass-g substrate -1 . Determine the cell productivity (g L -1 h -1 ) at steady state.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

1.00 is the correct answer to “A bacterial culture with an appropriate biomass composition of CH 1.8 O 0.5 N 0.2 is grown aerobically on a defined medium containing glucose as the sole carbon source and ammonia being the nitrogen source. In this fermentation, biomass is formed with a yield coefficient of 0.35 gram dry cell weight per gram of glucose and acetate is produced with a yield coefficient of 0.1 gram acetate per gram of glucose. The respiratory coefficient for the above culture will be”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

20,000 cycles h-1 is the correct answer to “Batch fermentation of glucose to ethanol yields a productivity of 4.5 g -1 L h -1 . If the yeast cell concentration in the fermentation broth is 5% (v/v) and the intracellular NAD + /NADH concentration in the yeast cells is 10 μM, the cycling rate of NAD + ⇌ NADH will be”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

600 cm2 and 6 × 107 cells is the correct answer to “A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (l) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 10 5 anchorage dependent cells per cm 2 , the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

7 is the correct answer to “A protein binds to phosphocellulose column at pH 7.0 and elutes at pH 8.0. If the protein has to be further purified on a DEAE Sephacel column, the binding buffer should have a pH of”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Hollow fibers is the correct answer to “Which one of the following monolayer culture systems have the highest surface area to medium ratio”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-2, Q-3, R-1, S-4 is the correct answer to “Match the downstream processes in Group I with the products in Group II . Group I Group II P. Solvent extraction 1. Lactic acid Q. Protein-A linked affinity chromatography 2. Penicillin R. Extractive distillation 3. Monoclonal antibody d. S. Salting out 4. Lipase”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Immobilization is the correct answer to “Which one of the following reactions is used for the purpose of recycling enzymes in bioprocesses”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Continued replacement of the bulk liquid containing the substrate is the correct answer to “The factor(s) likely to increase the rate of reaction catalyzed by a surface immobilized enzyme is/are”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

$$\frac{g}{{g - h}}$$ is the correct answer to “The unit for specific substrate consumption rate in a growing culture is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.