Bioprocess Engineering And Process Biotechnology — Part 2 MCQs with Explanations

Mole fraction of G + C is the correct answer to “Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs + DNA is given by the equation ρ = 1.66 + 0.098 X G + C , where X G + C denotes”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-2, Q-4, R-1, S-3 is the correct answer to “Match the instruments in Group I with their corresponding measurements in Group II . Group I Group II P. Manometer 1. Agitator speed Q. Rotameter 2. Pressure difference R. Tachometer 3. Cell number S. Hemocytometer 4. Air flow rate”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

All of the above is the correct answer to “Autoclaves are routinely used in laboratories for sterilization. They act by”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Both (A) and (R) are true and (R) is the correct reason for (A) is the correct answer to “Determine the correctness or otherwise of the following Assertion (A) and the Reason (R) . Assertion: In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously. Reason: Synchronous culture could be obtained by starving cells for essential nutrient components.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Q, R and S only is the correct answer to “In balanced growth phase of a cell, P. All components of a cell grow at the same rate. Q. Specific growth determined by cell number or cell mass would be the same. R. The growth rate is independent of substrate concentration. S. The growth rate decreases with decreasing substrate concentration.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P, Q and R is the correct answer to “Which of the following factors can influence the lag phase of a microbial culture in a batch fermenter? P. Inoculum size Q. Inoculum age R. Medium composition”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

nND is the correct answer to “Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given, N = rotations/time, D = diameter of impeller)”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-3, Q-4, R-2, S-1 is the correct answer to “Match the properties in Group I with the downstream operations in Group II . Group I Group II P. Size 1. Extraction Q. Density 2. Distillation R. Volatility 3. Filtration S. Solubility 4. Sedimentation”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

61 is the correct answer to “The diameters of a large and a small vessel are 1.62 m and 16.2 cm, respectively. The vessels are geometrically similar and operated under similar volumetric agitated power input. The mixing time in the small vessel was found to be 15 s. Determine the mixing time (in seconds) in the large vessel.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Internal reserves is the correct answer to “Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

R, S is the correct answer to “Which of the following statements are true about bioreactors? P. Continuous bioreactors provide less degree of control and uniform product quality than batch bioreactors. Q. Batch bioreactors are ideally suited for reaction with substrate inhibition. R. Choice of a bioreactor is dictated by kinetic considerations. S. Fed batch bioreactors are also called semi-batch bioreactors.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P, Q and S only is the correct answer to “Which of the following statements is always correct about an ideal chemostat? P. Substrate concentration inside the chemostat is equal to that in the exit stream. Q. Optimal dilution rate is lower than critical dilution rate. R. Biomass concentration increases with increase in dilution rate. S. Cell recirculation facilitates operation beyond critical dilution rate.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Only A and B of the above is the correct answer to “Which of the following statements applies to the operation of a fed-batch process”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

X = 0, S = S0, P = 0 is the correct answer to “Wash out (as defined by D = μ max ) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S 0 = substrate concentration in feed, P = product concentration in bioreactor).”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-3, Q-4, R-1, S-2 is the correct answer to “Match each parameter in Group I with the appropriate measuring device in Group II . Group I Group II P. Pressure 1. Photometer Q. Foam 2. Rotameter R. Turbidity 3. Diaphragm gauge S. Flow rate 4. Rubber sheathed electrode”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Culture agitation to maintain oxygen supply is the correct answer to “To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Both (A) and (R) are true and (R) is the correct reason for (A) is the correct answer to “Determine the correctness or otherwise of the following Assertion (A) and Reason (R) . Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation. Reason: Immobilization protects the plant cells from shear forces in the bioreactor.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

It provides low shear resistance to cells is the correct answer to “Which of the following statements is incorrect about immobilized plant cell cultures”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

μ < 0 is the correct answer to “Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-4, Q-1, R-2, S-3 is the correct answer to “Match the coefficients in Group I with their corresponding downstream processing Steps given in Group II . Group I Group II P. Sedimentation coefficient 1. Aqueous two-phase extraction Q. Partition coefficient 2. Ultrafiltration R. Rejection coefficient 3. Dialysis S. Activity coefficient 4. Centrifugation”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Substrate feed rate cycling is the correct answer to “In chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than maximum growth rate by”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P, Q and R only is the correct answer to “Substrate consumption in lag phase of microbial growth is primarily used for P. Turnover of the cell material. Q. Maintenance of intracellular pH. R. Motility. S. Increase in cell number.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

NADPH dehydrogenase is the correct answer to “Cibacron Blue dye affinity chromatography can be used for affinity purification of”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Biostat is the correct answer to “A culture vessel in which physical, physicochemical and physiological conditions, as well as cell concentration, are kept constant is known as”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

None of the above is the correct answer to “Inversion of sucrose by immobilized invertase follows a substrate inhibited kinetics. The reaction rate (v) in mol m -3 h -1 can be expressed as: $$v = \frac{{800[S]}}{{\left\{ {400 + 50[S] + {{[S]}^2}} \right\}}};$$ where [S] is the sucrose concentration. The immobilized invertase preparation is used in a CSTR with 100 mol m -3 sucrose concentration in the feed stream. If the reaction velocity passes through a maximum at [S] = 20 mol m -3 the feed flow rate for a reactor volume of 1 m 3 to get the maximum productivity from the reactor should be”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

$${\left( {\frac{{{D_1}}}{{{D_2}}}} \right)^2}$$ is the correct answer to “A bioreactor is scaled up based on equal impeller tip speed. Consider the following parameters for small and large bioreactors: Parameters Small bioreactor Large bioreactor Impeller speed N 1 N 2 Diameter of impeller D 1 D 2 Power consumption P 1 P 2 Assuming geometrical similarity and the bioreactors are operated in turbulent regime, what will be $$\frac{{{P_2}}}{{{P_1}}}$$”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

0.51 is the correct answer to “How many kilograms of ethanol is produced from 1 kilogram of glucose in ethanol fermentation”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P-3, Q-1, R-4, S-2 is the correct answer to “Match the bioreactor components in Group I with the most appropriate function given in Group II . Group I Group II P. Marine type impeller 1. Recirculation of medium Q. Draft tube 2. Aeration of medium R. Diaphragm valve 3. Animal cell cultivation S. Sparger 4. Sterile operation”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Q, S is the correct answer to “Immobilization of enzymes P. Increases the specificity of the enzyme for its reactants. Q. Facilitates reuse of the enzyme in batch reactions. R. Makes it unsuitable for its use in a continuous reactor system. S. Decreases the operational cost of the industrial process.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

65 g L-1 is the correct answer to “A bacterial culture having a specific oxygen uptake rate of 5 mmol O 2 (g DCW) -1 hr -1 is being grown aerobically in a fed-batch bioreactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s -1 for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg mL -1 ). The maximum density to which the cells can be grown in the fed-batch process without the growth being limited by oxygen transfer, is approximately”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Only B and C of the above is the correct answer to “The limiting factor in the production of large quantities of ethanol as biofuel is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

0.41 is the correct answer to “If the fractional recovery at each step of unit operation is 0.8, the recovery after 4 steps will be”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P, Q is the correct answer to “Diauxic pattern of biomass growth is associated with P. Multiple lag phases. Q. Sequential utilization of multiple substrates. R. Simultaneous utilization of multiple substrates. S. Absence of lag phase.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Q, P, R, S is the correct answer to “Downstream processing of an industrial process yielded a highly purified bioactive protein. This protein was subjected to cleavage by trypsin. Chromatographic separation of products resulted in 4 peptides (P, Q, R, S) with the following amino acid sequences. P. Phe-Val-Met-Val-Arg Q. Ala-Ala-Try-Gly-Lys R. Val-Phe-Met-Ala-Gly-Lys S. Phe-Gly-Try-Ser-Thr Chemical cleavage of the same protein with cyanogen bromide and chromatographic separation resulted in three peptides (i, ii, iii) with the following sequences (i) Ala-Gly-Lys-Phe-Gly-Try-Ser-Thr (ii) Ala-Ala-Try-Gly-Lys-Phe-Val-Met (iii) Val-Arg-Val-Phe-Met The order of the peptides that gives the primary structure of the original protein is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Ion-exchange chromatography is the correct answer to “Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Sparger is the correct answer to “Aeration in a bioreactor is provided by”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

0 (zero) is the correct answer to “The specific growth rate (μ) of a microorganism in death phase is”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

P, S is the correct answer to “Immobilization of enzymes using entrapment method requires P. Photosensitive polyethylene glycol dimethacrylate. Q. CNBr activation of sepharose. R. Polyfunctional reagent like hexamethylene diisocyanate. S. Radiation of polyvinyl alcohol.”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

Physical bonding by flocculation is the correct answer to “Which of the following techniques is not ideal for immobilizing cell-free enzymes”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.

(A) is true but (R) is false is the correct answer to “Determine the correctness or otherwise of the following Assertion (A) and Reason (R) . Assertion: Bacterial growth is called synchronous when majority of the cells are in same stage of the bacterial cell cycle. Reason: Synchronous culture can be obtained by growing bacteria in an enriched medium”. This question is standard in competitive examination syllabi for Bioprocess Engineering And Process Biotechnology.