Computational Tools In Biotechnology — Part 1 MCQs with Explanations

Homology modeling is the correct answer to “The method used for prediction of three-dimensional structure of a protein from known structure(s) of one or more related proteins is”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Blastx is the correct answer to “Which one of the following BLAST search programs is used to identify homologs of a genomic DNA query in a protein sequence database”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Clastal W is the correct answer to “The multiple sequence alignment tool is . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

15 and 3 is the correct answer to “How many rooted and unrooted phylogenetic trees, respectively, are possible with four different sequences”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

NBRF is the correct answer to “Identify the file format given below: >P1; JMFD Protein X - Homo sapiens MKALT ARQQEVFDLIRDHISRTLRQQGDWL”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Lead is the correct answer to “The compound that has properties to become a drug is known as . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

TBLASTX is the correct answer to “Which one of the following tools is used to compare all the possible six-open reading frames of a given nucleotide query sequence with all the available six-open reading frames of the nucleotide sequence database”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Computational tool for sequence homology searching and alignment is the correct answer to “In bioinformatics, the term 'BLAST' refers to”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Both (A) and (R) are true and (R) is the correct reason for (A) is the correct answer to “Determine the correctness or otherwise of the following Assertion (A) and the Reason (R) . Assertion: Ab initio gene finding algorithms that predict protein coding genes in eukaryotic genomes are not completely accurate. Reason: Eukaryotic splice sites are difficult to predict.”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Probability from a normal distribution is the correct answer to “While searching a database for similar sequences, E-value does not depend on the”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

CLUSTAL is the correct answer to “The most widely used program for multiple sequence alignment is”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Phylogenetics is the correct answer to “The study of evolutionary relationships is known as”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

BLAST is the correct answer to “Program used for essentially local similarity search is”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

16 is the correct answer to “The length of the minimum unique stretch of DNA sequence that can be found only once in a 3 billion base pairs long genome is”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Q and R only is the correct answer to “Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm? P. A larger E-value indicates higher sequence similarity. Q. E-value < 10 -10 indicates sequence homology. R. A higher BLAST score indicates higher sequence similarity. S. E-value > 10 -10 indicates sequence homology.”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

PROSITE is the correct answer to “Which one of the following is a database of protein sequence motifs”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

SGD is the correct answer to “Which of the following is a model organism database”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Single database entry for DNA/Protein is the correct answer to “Accession number is a unique identification assigned to a”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

GEO is the correct answer to “An example of a derived protein structure database is”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

PAM 40 or BLOSUM 80 is the correct answer to “The suitable substitution matrix to align closely related sequences is”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

P and R only is the correct answer to “Identify the character-based method(s) used for the construction of a phylogenetic tree. P. Maximum parsimony Q. Neighbor joining R. Maximum likelihood S. Bootstrapping”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

PHYLIN is the correct answer to “An example of a program for constructing a phylogenetic tree”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Both [A] and [R] are true and [R] is the correct reason for [A] is the correct answer to “Amino acid sequences of cytochrome c and ribulose 5-phosphate epimerase from 40 organisms were chosen and phylogenetic trees were obtained for each of these two protein families. Determine the correctness or otherwise of the following Assertion [A] and the Reason [R] . Assertion [A]: The two trees will not be identical. Reason [R]: The nature and frequency of mutations in the two families are different.”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

P-2, Q-4, R-1, S-3 is the correct answer to “Match the items in Group I with an appropriate description in Group II . Group I Group II P. UPGMA 1. Protein sequence database Q. CLUSTAL 2. Phylogenetic analysis R. SWISS-PROT 3. 3-D structure visualization S. RasMol 4. Multiple sequence alignment”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

15 is the correct answer to “Number of unrooted trees in a phylogeny of five sequences is”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

EMBL is the correct answer to “Which one of these is a nucleotide sequence data base”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Model organism database is the correct answer to “The term "Flybase" is a . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

k-tuple analysis is the correct answer to “The algorithm for BLAST is based on”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Exocytosis is the correct answer to “The process in which macromolecules are released out of cells is known as . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Primary database for macromolecules is the correct answer to “PDB is a . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

R, S is the correct answer to “For prediction of three-dimensional structure of protein P. Homology mode tries many possible alignments. Q. Threading first identifies homologues. R. Threading evaluates many rough models. S. Homology modeling optimizes one model.”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

A ribosomal RNA of small ribosomal subunit is the correct answer to “Carl Woese used the gene sequence of which one of the following for phylogenetic taxonomy of prokaryotes”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Entrez is the correct answer to “The retrivel and search tool of the NCBI is . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Bootstrap is the correct answer to “Which one of the following is not an algorithm for building phylogenetic trees”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Bootstrap is the correct answer to “Which one of the following methods is used to test the significance of a predicted phylogeny”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

PAM100, PAM120, PAM250 is the correct answer to “The amino acid substitution matrices in decreasing order of stringency for comparing protein sequences are”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Insilico is the correct answer to “Somethong performed on computer or computer simulation is . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Set of entire proteins in a cell is the correct answer to “Proteomics is the study of . . .”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

P-2, Q-4, R-3, S-1 is the correct answer to “Match the entries in the Group I with the entries in Group II . Group I Group II P. Threading 1. Gene duplication Q. FASTA 2. Fold prediction R. Profile 3. HMM S. Paralogs 4. k-tuple”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.

Both (A) and (R) are true and (R) is not the correct reason for (A) is the correct answer to “Determine the correctness or otherwise of the following Assertion (A) and Reason (R) . Assertion: UPGMA method produces ultrametric tree. Reason: Sequence alignment is converted into evolutionary distances in UPGMA method.”. This question is standard in competitive examination syllabi for Computational Tools In Biotechnology.