Enzyme Engineering In Biotechnology — Part 1 MCQs with Explanations

Chemical treatment is the correct answer to “Which of the following is the best method for isolating enzymes from cell free extract”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

False is the correct answer to “Absolute specificity, group specificity and optical specificity are observed by chemical catalyst.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Ligases is the correct answer to “Which of the following enzyme is used to join two DNA molecule”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

False is the correct answer to “The rate of enzyme catalyzed reaction decreases with an increase in ionic strength if the charges are identical.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Sodium dodecyl sulphate (SDS) is the correct answer to “. . . . . . . . is an example of anionic detergent.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Consumed in reaction is the correct answer to “Which among them is not attribute of enzymes:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Product is the correct answer to “The rate equation for . . . . . . . . inhibition is given by $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m}\left( {1 + {{\left[ P \right]} \over {{K_p}}}} \right) + \left[ S \right]}}.$$”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Half-life is the correct answer to “The equation $${t_{{1 \over 2}}} = {{0.693} \over {{k_d}}}$$ represents . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Induced fit model is the correct answer to “Koshland proposed which model:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Histidase is the correct answer to “If enzyme code EC 2.6.1.1 belongs to aspartate amino transferase, then the code EC 4.3.1.3 belongs to which enzyme”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Freezing is the correct answer to “. . . . . . . . technique is nothing but a cold or psychrophilic shock.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

SI nuclease is the correct answer to “Selective degradation of single stranded DNA is carried out by the enzyme”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Epimerases is the correct answer to “Which of the following belongs to the class of isomerases”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Terminal nucleotidyl transferase is the correct answer to “The enzyme that adds mononucleotide triphosphates to the 3' OH group of a DNA fragment is”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

RNase-H is the correct answer to “The RNA strand in the RNA-DNA hybrid is removed by”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Proton is the correct answer to “. . . . . . . . is responsible for maintaining biologically native structure of an enzyme and even nucleic acid.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Enzymes is the correct answer to “. . . . . . . . mediates the synthetic and degradative reactions carried out by living organism.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Succinic dehydrogenase by malonic acid is the correct answer to “Which one among them is the example of competitive inhibition of an enzyme:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Unstable nature of enzymes is the correct answer to “Major drawback of utilizing enzymes is . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Ultrasonication is the correct answer to “. . . . . . . . is a method which is highly used for lab scale procedures than large scale work.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Amino acids is the correct answer to “Enzymes are the polymers of . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Alkaline phosphatase is the correct answer to “Which is the enzyme used to remove phosphate group from the 5' end of the DNA”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

4.62*103 is the correct answer to “If the rate of denaturation of enzyme Y is 1.5*10 -4 /min, then the half-life of enzyme Y is . . . . . . . . mins.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Often a vitamin is the correct answer to “The coenzyme is:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Arrhenius equation is the correct answer to “What does the equation, $$k = A{e^{{{ - \Delta G} \over {RT}}}}$$ represent”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

False is the correct answer to “Uncompetitive inhibition is most noticeable at low substrate concentration and can be overcome at high substrate concentration.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Mention of cofactors is the correct answer to “Which of the following rule was not given by the enzyme commission”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Suitable density gradient is the correct answer to “Which of the following should be implemented in case of extracting conjugating enzymes”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Starch is the correct answer to “Diastase takes part in digestion of which one:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

pKa is the correct answer to “The pH at which half the groups of a compound are ionized is referred to as . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Extraction procedure is the correct answer to “Which of the following is not a reason for selected source to be used readily for the production of enzymes”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

EDTA is the correct answer to “Which of the following chemical is not used to isolate enzymes from cell free extract”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

EDTA is the correct answer to “. . . . . . . . is a divalent chelator which can be used to extract enzymes from different sources.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Hydrolases is the correct answer to “Glycosidases, lipases and proteases belong to which class of enzymes”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Homogenization is the correct answer to “Which of the following is not a technique for the extraction of enzymes by chemical methods”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Ligase is the correct answer to “An enzyme that joins the ends of two strands of nucleic acid is:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Competitive inhibition is the correct answer to “Blocking of enzyme action by blocking its active site is called as:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Ultrasonication is the correct answer to “The procedure in which cell lysis occur because of shock waves which are formed due to collapses occurring in buffered solution containing cells is referred to as . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Tween is the correct answer to “Which one of the following is not an example for ionic-detergent”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.

Reducing the activation energy is the correct answer to “The general mechanism is that an enzyme acts by:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.