Enzyme Engineering In Biotechnology — Part 2 MCQs with Explanations
Practice high-yield Biotechnology Engineering MCQs on Enzyme Engineering In Biotechnology — Part 2 with worked academic solutions for PPSC, FPSC, NIH, NTS.
Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40
Verified Practice Questions
Interactive test mode with detailed academic worked solutions
Live Progress|0/40 answered
✓0 correct
✗0 wrong
0% score
🎉 Set complete! All 40 questions answered.
Question 1 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
This enzyme was first isolated and purified in the form of crystals:
Correct Answer: Option A — Urease
Detailed Academic Explanation:
Urease is the correct answer to “This enzyme was first isolated and purified in the form of crystals:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 2 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
. . . . . . . . are very efficient catalyst as compared to conventional catalyst.
Correct Answer: Option A — Enzymes
Detailed Academic Explanation:
Enzymes is the correct answer to “. . . . . . . . are very efficient catalyst as compared to conventional catalyst.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 3 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
. . . . . . . . is the main contaminant present in the resultant free extract from cells.
Correct Answer: Option D — Nucleic acids
Detailed Academic Explanation:
Nucleic acids is the correct answer to “. . . . . . . . is the main contaminant present in the resultant free extract from cells.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 4 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The class of enzymes which contains extensive group of enzymes are . . . . . . . .
Correct Answer: Option B — Oxidoreductases
Detailed Academic Explanation:
Oxidoreductases is the correct answer to “The class of enzymes which contains extensive group of enzymes are . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 5 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is produced with the combination of apoenzyme and coenzyme:
Correct Answer: Option A — Holoenzyme
Detailed Academic Explanation:
Holoenzyme is the correct answer to “Which of the following is produced with the combination of apoenzyme and coenzyme:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 6 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is an example for Oxidoreductases?
Correct Answer: Option A — Glucose oxidase
Detailed Academic Explanation:
Glucose oxidase is the correct answer to “Which of the following is an example for Oxidoreductases”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 7 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is the non-protein component of the enzyme?
Correct Answer: Option D — All of these
Detailed Academic Explanation:
All of these is the correct answer to “Which of the following is the non-protein component of the enzyme”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 8 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is a thermo stable DNA polymerase
Correct Answer: Option D — All of these
Detailed Academic Explanation:
All of these is the correct answer to “Which of the following is a thermo stable DNA polymerase”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 9 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following enzyme cannot be extracted from liver?
Correct Answer: Option B — Lactose synthase
Detailed Academic Explanation:
Lactose synthase is the correct answer to “Which of the following enzyme cannot be extracted from liver”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 10 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In the human body optimum temperature for enzymatic activities is:
Correct Answer: Option A — 37-degree celsius
Detailed Academic Explanation:
37-degree celsius is the correct answer to “In the human body optimum temperature for enzymatic activities is:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 11 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Mg +2 is an inorganic activator for enzymes:
Correct Answer: Option A — Phosphatase
Detailed Academic Explanation:
Phosphatase is the correct answer to “Mg +2 is an inorganic activator for enzymes:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 12 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
. . . . . . . . inhibition represent the following rate equation. $$V = {{\left( {{{{V_{\max }}} \over {1 + {{\left[ I \right]} \over {{K_i}}}}}} \right)\left[ S \right]} \over {{K_m} + \left[ S \right]}}$$
Correct Answer: Option D — Non-competitive inhibition
Detailed Academic Explanation:
Non-competitive inhibition is the correct answer to “. . . . . . . . inhibition represent the following rate equation. $$V = {{\left( {{{{V_{\max }}} \over {1 + {{\left[ I \right]} \over {{K_i}}}}}} \right)\left[ S \right]} \over {{K_m} + \left[ S \right]}}$$”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 13 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Alkali treatment is not advisable for which of the following?
Correct Answer: Option A — Extracellular enzymes
Detailed Academic Explanation:
Extracellular enzymes is the correct answer to “Alkali treatment is not advisable for which of the following”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 14 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Eco R1 is a
Correct Answer: Option A — Endonuclease
Detailed Academic Explanation:
Endonuclease is the correct answer to “Eco R1 is a”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 15 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following enzyme is used to cut DNA molecule internally
Correct Answer: Option B — Restriction endonuclease
Detailed Academic Explanation:
Restriction endonuclease is the correct answer to “Which of the following enzyme is used to cut DNA molecule internally”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 16 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
. . . . . . . . is a compound which is frequently used for DNA removal.
Correct Answer: Option B — Protamine sulphate
Detailed Academic Explanation:
Protamine sulphate is the correct answer to “. . . . . . . . is a compound which is frequently used for DNA removal.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 17 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which among them is a cofactor:
Correct Answer: Option C — Both A and B
Detailed Academic Explanation:
Both A and B is the correct answer to “Which among them is a cofactor:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 18 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
What kind of specificity does glucose oxidase exhibit?
Correct Answer: Option A — Absolute specificity
Detailed Academic Explanation:
Absolute specificity is the correct answer to “What kind of specificity does glucose oxidase exhibit”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 19 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
All proteins are not enzymes.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “All proteins are not enzymes.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 20 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Mortar and pestle, french press and homogenizer are widely used to lyse the cells by ultrasonication.
Correct Answer: Option B — False
Detailed Academic Explanation:
False is the correct answer to “Mortar and pestle, french press and homogenizer are widely used to lyse the cells by ultrasonication.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 21 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Enzymes discriminate between similar parts of same molecules. This is termed as . . . . . . . .
Correct Answer: Option C — Regiospecificity
Detailed Academic Explanation:
Regiospecificity is the correct answer to “Enzymes discriminate between similar parts of same molecules. This is termed as . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 22 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The enzyme minus its coenzyme known as:
Correct Answer: Option A — Apoenzyme
Detailed Academic Explanation:
Apoenzyme is the correct answer to “The enzyme minus its coenzyme known as:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 23 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Zn +2 is an inorganic activator for enzymes:
Correct Answer: Option C — Chymotrypsin
Detailed Academic Explanation:
Chymotrypsin is the correct answer to “Zn +2 is an inorganic activator for enzymes:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 24 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Freezing technique is not preferred unless otherwise there is a need.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “Freezing technique is not preferred unless otherwise there is a need.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 25 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The . . . . . . . . inhibition gives the following rate equation. $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m} + \left[ S \right]\left( {1 + {{\left[ S \right]} \over {{K_s}}}} \right)}}$$
Correct Answer: Option A — Substrate
Detailed Academic Explanation:
Substrate is the correct answer to “The . . . . . . . . inhibition gives the following rate equation. $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m} + \left[ S \right]\left( {1 + {{\left[ S \right]} \over {{K_s}}}} \right)}}$$”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 26 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is cationic detergent?
Correct Answer: Option B — Cetyl triethyl ammonium bromide
Detailed Academic Explanation:
Cetyl triethyl ammonium bromide is the correct answer to “Which of the following is cationic detergent”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 27 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Enzymes are amphoteric molecules.
Correct Answer: Option A — True
Detailed Academic Explanation:
True is the correct answer to “Enzymes are amphoteric molecules.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 28 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Zymogen or proenzyme is a:
Correct Answer: Option C — Enzyme precursor
Detailed Academic Explanation:
Enzyme precursor is the correct answer to “Zymogen or proenzyme is a:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 29 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The following rate equation represents . . . . . . . . inhibition. $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m}\left( {1 + {I \over {{K_i}}}} \right) + \left[ S \right]}}$$
Correct Answer: Option A — Competitive
Detailed Academic Explanation:
Competitive is the correct answer to “The following rate equation represents . . . . . . . . inhibition. $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m}\left( {1 + {I \over {{K_i}}}} \right) + \left[ S \right]}}$$”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 30 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Enzymes are made Up of:
Correct Answer: Option B — Proteins
Detailed Academic Explanation:
Proteins is the correct answer to “Enzymes are made Up of:”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 31 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The techniques which adopts the hypertonic and hypotonic condition for cell lysis is referred to as . . . . . . . .
Correct Answer: Option C — Osmotic shock
Detailed Academic Explanation:
Osmotic shock is the correct answer to “The techniques which adopts the hypertonic and hypotonic condition for cell lysis is referred to as . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 32 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Phytanase can be extracted only from . . . . . . . . species.
Correct Answer: Option B — Prokaryote
Detailed Academic Explanation:
Prokaryote is the correct answer to “Phytanase can be extracted only from . . . . . . . . species.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 33 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Catalyst discovered from biological sources are referred to as . . . . . . . .
Correct Answer: Option C — Enzymes
Detailed Academic Explanation:
Enzymes is the correct answer to “Catalyst discovered from biological sources are referred to as . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 34 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Enzymes that catalyze the transfer of atom or group between two molecules is known as . . . . . . . .
Correct Answer: Option B — Transferases
Detailed Academic Explanation:
Transferases is the correct answer to “Enzymes that catalyze the transfer of atom or group between two molecules is known as . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 35 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
In . . . . . . . . treatment, certain molecules interact through ionic or Vander wall forces which leads to solubilization of phospholipids and lipoprotein which in turn, increases membrane permeability directing to cell lysis.
Correct Answer: Option C — Detergent
Detailed Academic Explanation:
Detergent is the correct answer to “In . . . . . . . . treatment, certain molecules interact through ionic or Vander wall forces which leads to solubilization of phospholipids and lipoprotein which in turn, increases membrane permeability directing to cell lysis.”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 36 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is an RNA dependent DNA synthetase
Correct Answer: Option C — Reverse transcriptase
Detailed Academic Explanation:
Reverse transcriptase is the correct answer to “Which of the following is an RNA dependent DNA synthetase”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 37 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The pH at which the net charge on the enzyme molecule is zero is called . . . . . . . .
Correct Answer: Option C — Isoelectric point
Detailed Academic Explanation:
Isoelectric point is the correct answer to “The pH at which the net charge on the enzyme molecule is zero is called . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 38 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as . . . . . . . .
Correct Answer: Option B — Freezing
Detailed Academic Explanation:
Freezing is the correct answer to “The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as . . . . . . . .”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 39 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
Which of the following is an advantage of enzymes over chemical catalysts?
Correct Answer: Option A — High productivity, less material cost
Detailed Academic Explanation:
High productivity, less material cost is the correct answer to “Which of the following is an advantage of enzymes over chemical catalysts”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
Question 40 of 40
★ PPSC
★ FPSC
★ PPSC
★ FPSC
What happens to V max and K m in case of uncompetitive inhibition when I = K i '?
Correct Answer: Option A — Lowers to 0.5 Vmax and 0.5 Km
Detailed Academic Explanation:
Lowers to 0.5 Vmax and 0.5 Km is the correct answer to “What happens to V max and K m in case of uncompetitive inhibition when I = K i '”. This question is standard in competitive examination syllabi for Enzyme Engineering In Biotechnology.
💡Key Conceptual Anchor:Enzyme Engineering In Biotechnology
✓Link copied to clipboard
Continue Your Practice
Analytical Tools In Biotechnology — Part 1
Master the next curriculum module with 40 exam-standard MCQs.
💡Examiner's Advisory for Biotechnology Engineering
In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.