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Enzyme Engineering In Biotechnology — Part 2 MCQs with Explanations

Practice high-yield Biotechnology Engineering MCQs on Enzyme Engineering In Biotechnology — Part 2 with worked academic solutions for PPSC, FPSC, NIH, NTS.

Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40

Verified Practice Questions

Interactive test mode with detailed academic worked solutions

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Question 1 of 40
★ PPSC ★ FPSC

This enzyme was first isolated and purified in the form of crystals:

Question 2 of 40
★ PPSC ★ FPSC

. . . . . . . . are very efficient catalyst as compared to conventional catalyst.

Question 3 of 40
★ PPSC ★ FPSC

. . . . . . . . is the main contaminant present in the resultant free extract from cells.

Question 4 of 40
★ PPSC ★ FPSC

The class of enzymes which contains extensive group of enzymes are . . . . . . . .

Question 5 of 40
★ PPSC ★ FPSC

Which of the following is produced with the combination of apoenzyme and coenzyme:

Question 6 of 40
★ PPSC ★ FPSC

Which of the following is an example for Oxidoreductases?

Question 7 of 40
★ PPSC ★ FPSC

Which of the following is the non-protein component of the enzyme?

Question 8 of 40
★ PPSC ★ FPSC

Which of the following is a thermo stable DNA polymerase

Question 9 of 40
★ PPSC ★ FPSC

Which of the following enzyme cannot be extracted from liver?

Question 10 of 40
★ PPSC ★ FPSC

In the human body optimum temperature for enzymatic activities is:

Question 11 of 40
★ PPSC ★ FPSC

Mg +2 is an inorganic activator for enzymes:

Question 12 of 40
★ PPSC ★ FPSC

. . . . . . . . inhibition represent the following rate equation. $$V = {{\left( {{{{V_{\max }}} \over {1 + {{\left[ I \right]} \over {{K_i}}}}}} \right)\left[ S \right]} \over {{K_m} + \left[ S \right]}}$$

Question 13 of 40
★ PPSC ★ FPSC

Alkali treatment is not advisable for which of the following?

Question 14 of 40
★ PPSC ★ FPSC

Eco R1 is a

Question 15 of 40
★ PPSC ★ FPSC

Which of the following enzyme is used to cut DNA molecule internally

Question 16 of 40
★ PPSC ★ FPSC

. . . . . . . . is a compound which is frequently used for DNA removal.

Question 17 of 40
★ PPSC ★ FPSC

Which among them is a cofactor:

Question 18 of 40
★ PPSC ★ FPSC

What kind of specificity does glucose oxidase exhibit?

Question 19 of 40
★ PPSC ★ FPSC

All proteins are not enzymes.

Question 20 of 40
★ PPSC ★ FPSC

Mortar and pestle, french press and homogenizer are widely used to lyse the cells by ultrasonication.

Question 21 of 40
★ PPSC ★ FPSC

Enzymes discriminate between similar parts of same molecules. This is termed as . . . . . . . .

Question 22 of 40
★ PPSC ★ FPSC

The enzyme minus its coenzyme known as:

Question 23 of 40
★ PPSC ★ FPSC

Zn +2 is an inorganic activator for enzymes:

Question 24 of 40
★ PPSC ★ FPSC

Freezing technique is not preferred unless otherwise there is a need.

Question 25 of 40
★ PPSC ★ FPSC

The . . . . . . . . inhibition gives the following rate equation. $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m} + \left[ S \right]\left( {1 + {{\left[ S \right]} \over {{K_s}}}} \right)}}$$

Question 26 of 40
★ PPSC ★ FPSC

Which of the following is cationic detergent?

Question 27 of 40
★ PPSC ★ FPSC

Enzymes are amphoteric molecules.

Question 28 of 40
★ PPSC ★ FPSC

Zymogen or proenzyme is a:

Question 29 of 40
★ PPSC ★ FPSC

The following rate equation represents . . . . . . . . inhibition. $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m}\left( {1 + {I \over {{K_i}}}} \right) + \left[ S \right]}}$$

Question 30 of 40
★ PPSC ★ FPSC

Enzymes are made Up of:

Question 31 of 40
★ PPSC ★ FPSC

The techniques which adopts the hypertonic and hypotonic condition for cell lysis is referred to as . . . . . . . .

Question 32 of 40
★ PPSC ★ FPSC

Phytanase can be extracted only from . . . . . . . . species.

Question 33 of 40
★ PPSC ★ FPSC

Catalyst discovered from biological sources are referred to as . . . . . . . .

Question 34 of 40
★ PPSC ★ FPSC

Enzymes that catalyze the transfer of atom or group between two molecules is known as . . . . . . . .

Question 35 of 40
★ PPSC ★ FPSC

In . . . . . . . . treatment, certain molecules interact through ionic or Vander wall forces which leads to solubilization of phospholipids and lipoprotein which in turn, increases membrane permeability directing to cell lysis.

Question 36 of 40
★ PPSC ★ FPSC

Which of the following is an RNA dependent DNA synthetase

Question 37 of 40
★ PPSC ★ FPSC

The pH at which the net charge on the enzyme molecule is zero is called . . . . . . . .

Question 38 of 40
★ PPSC ★ FPSC

The technique in which the cells are subjected to cold temperature of about -15° C to -25° C is referred to as . . . . . . . .

Question 39 of 40
★ PPSC ★ FPSC

Which of the following is an advantage of enzymes over chemical catalysts?

Question 40 of 40
★ PPSC ★ FPSC

What happens to V max and K m in case of uncompetitive inhibition when I = K i '?

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In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.

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