Methods And Techniques In Applied Biology MCQs with Explanations

Location of restriction sites in a particular gene is the correct answer to “Which of the following would not possible to address using a Northern Blot”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

DNA is the correct answer to “In southern blotting . . . . . . . . is separated by gel electrophoresis”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

256 bp is the correct answer to “A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

To add base at the 3' end of the DNA is the correct answer to “Terminal transferase is used”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

The plasmid contains the gene encoding β-lactamase is the correct answer to “Many plasmids have Ampicillin marker. This implies”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Thymidine is the correct answer to “DNA synthesis can be measured by estimating incorporation of radio-labelled”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Ethidium bromide can be used to visualize the DNA is the correct answer to “In agarose gel electrophoresis”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Cosmid is the correct answer to “A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Alwine et al is the correct answer to “Northern blotting is developed by”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

1 Kbp is the correct answer to “A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Synthesis of purine deoxynucleotides by de novo path way is the correct answer to “Cells deficient in hypoxanthine guanine phospho-ribosyl transferase (HGPRT)”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Entry of the vector containing the cDNA and the drug resistance genes into the host cell renders the latter identifiable as it is now resistant to antibiotic drugs is the correct answer to “Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

The ability to 'sequence' both strands of the DNA duplex simultaneously is the correct answer to “There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

The nuclei of somatic cells contain all the genetic information present in a fertilized egg is the correct answer to “The ability to clone an adult animal from the nucleus of a differentiated somatic cell proves that”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

DNA between the two cells should be compatible is the correct answer to “For protoplast fusion to be successful in plant cells.”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Western blotting is the correct answer to “The process used for separation of protein in polyacrylamide gel is called:”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Genetic engineering is the correct answer to “The technique which involves addition or deletions of genes is”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

A and B both is the correct answer to “In Southern blotting, DNA is fixed on”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Option "$$\frac{{{\text{Distance from origin run by the compound}}}}{{{\text{Distance from origin run by the solvent}}}}$$" is the correct answer for this question under Methods And Techniques In Applied Biology.

10-9 g/ml is the correct answer to “The sensitivity of ELISA is”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Synthesis of monoclonal antibodies is the correct answer to “Hybridoma technology is used in”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Restriction enzymes are exonucleases rather than endonucleases is the correct answer to “Which statement about restriction enzymes are false”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Auxotrophic bacteria is the correct answer to “Free amino acids can be determined with the help of”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

The DNA molecule has cohesive terminal ends is the correct answer to “If the linear double stranded genomic DNA of bacteriophage T4 is heated and cooled, one gets both linear and circular molecules because,”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

XmaIII (C/GGCGG) is the correct answer to “The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

It can identify genes associated with disease resistance, growth capacity, etc is the correct answer to “The genome sequencing of rice is important because”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

a-3, b-1, c-2 is the correct answer to “Match List-I (cloning vector) with List-II (character) and select the correct answer: List-I: Cloning vector List-II: Character a. Yeast episomal plasmid (YEp) 1. Integrates with host chromosome for replication b. Yeast integrative plasmid (YIp) 2. Carries a chromosomal origin of replication c. Yeast replicative plasmid (YRp) 3. Carries origin of replication of the 2 mm plasmid”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

DNA microarray is the correct answer to “Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

3'-end labelling with polynucleotide kinase is the correct answer to “Which of the following are not valid methods of labelling duplex DNA”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

A phosphodiester bond within a specific sequence is the correct answer to “Restriction endonucleases hydrolyzes polynucleotide from”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Alkaline phosphatase is the correct answer to “ELISA is used to detect viruses where the key reagent is”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

BT toxin gene is the correct answer to “Genetically engineered male sterile crop plants may be produced by inserting”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

1 : 26 is the correct answer to “Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

All of the above is the correct answer to “Which of the following is a desirable characteristic for a cloning plasmid”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

An error in RNA splicing that mistakenly removes an exon during RNA processing is the correct answer to “Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Fed-batch fermentation is the correct answer to “Which of the following is a semi-continuous fermentation in which only a small amount of substrate is available at the beginning of the process”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.

Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector is the correct answer to “Which statement correctly describes sequential steps in cDNA cloning”. This question is standard in competitive examination syllabi for Methods And Techniques In Applied Biology.