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Microbial Biotechnology MCQs with Explanations

Practice high-yield Biotechnology Engineering MCQs on Microbial Biotechnology with worked academic solutions for PPSC, FPSC, NIH, NTS.

Questions
33
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
40 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
33

Verified Practice Questions

Interactive test mode with detailed academic worked solutions

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Question 1 of 33
★ PPSC ★ FPSC

The precursor for penicillin-G biosynthesis during fermentation process is

Question 2 of 33
★ PPSC ★ FPSC

Which of the following eukaryotic organisms has been proven to be of great industrial importance?

Question 3 of 33
★ PPSC ★ FPSC

Oils rich in PUFA are not desirable for biodiesel production because

Question 4 of 33
★ PPSC ★ FPSC

Match the products in Group I with their ppossible applications in Group II . Group I Group II P. Erythropoietin 1. Blood clot Q. Anti-fibrin 99 2. Binding and transport of iron R. Collagenase 3. Anaemia S. S. Transferrin 4. Animal cell separation

Question 5 of 33
★ PPSC ★ FPSC

Match the recombinant products in Group I with their therapeutic applications in Group II . Group I Group II P. Human growth hormone 1. Pituitary dwarfism Q. Platelet growth factor 2. Chemotherapy induced thrombocytopenia R. Factor VIII 3. Hemophilia S. Erythropoietin 4. Anemia associated with chronic renal failure

Question 6 of 33
★ PPSC ★ FPSC

Identify the statement that is not correct

Question 7 of 33
★ PPSC ★ FPSC

Examine the data given below in the table on purification of a protein X. Step Volume (mL) Protein Concentration (mg mL -1) Enzyme Activity (unit mL -1 ) Crude cell-free extract 500 12.0 5.0 Ammonium sulfate precipitation 125 3.0 11.0 Ion-exchange chromatography 10 9.0 75.0 The yield percent and purification factor, respectively, at the end of the experiment will be approximately

Question 8 of 33
★ PPSC ★ FPSC

Match the activity spectrum of the following antibiotics. Group I Group II P. Actinomycin D 1. Antifungal Q. Daunorubicin 2. Antituberculosis R. Rifamycin 3. Antitumor S. Griseofulvin 4. Antiprotozoal

Question 9 of 33
★ PPSC ★ FPSC

Determine the correctness or otherwise of the following Assertion (A) and Reason (R) . Assertion: Cell mass yield of a methylotrophic yeast is more on methanol compared to glucose. Reason: Methanol has a greater degree of reductance compared to glucose.

Question 10 of 33
★ PPSC ★ FPSC

Match the products in Group I with their producer organisms given in Group II . Group I Group II P. Ethanol 1. Streptomyces orientalis Q. L-Lysine 2. Saccharomyces cerevisiae R. Biopesticide 3. Corynebacterium glutanicum S. Vancomycin 4. Bacillus thuringiensis

Question 11 of 33
★ PPSC ★ FPSC

Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria?

Question 12 of 33
★ PPSC ★ FPSC

Match the industrial application of the following enzymes. Group I Group II P. Penicillinase 1. Pharmaceutical Q. Pectinase 2. Leather R. Trypsin 3. Wine S. Rennin 4. Dairy

Question 13 of 33
★ PPSC ★ FPSC

Match the commercial microbial sources in Group I with the products in Group II . Group I Group II P. Corynebacterium lilium 1. 2, 3-Butane di-ol Q. Klebsiella oxytoca 2. Poly-β-hydroxybutyric acid R. Aspergillus niger 3. Glutamic acid S. Alcaligenes eutrophus 4. Citric acid

Question 14 of 33
★ PPSC ★ FPSC

Match enzymes in Group I with their corresponding industrial application in Group II . Group I Group II P. Amylase 1. Laundry detergent Q. Invertase 2. Fruit juice clarification R. Pectinase 3. Liquefaction of sucrose S. Xylanase 4. Pulp and paper processing

Question 15 of 33
★ PPSC ★ FPSC

In secondary metabolism, two distinct phases - trophophase and idiophase refer, respectively, to

Question 16 of 33
★ PPSC ★ FPSC

The two columns given below indicate some of the fermentation products and the microbial cultures used for their production. Identify the correct set of groups from the four options. Fermentation products Microbial cultures P. Ethanol 1. Aspergillus niger Q. Streptomycin 2. Zymomonas mobilis R. Citric acid 3. Streptomyces griseus S. Cellulase 4. Trichoderma reesei

Question 17 of 33
★ PPSC ★ FPSC

Match the following marker genes in Group I with suitable selecting agent in Group II . Group I Group II P. nptII 1. Glyphosate Q. aroA 2. Phosphinothricin R. hpt 3. Kanamycin S. bar 4. Hygromycin B

Question 18 of 33
★ PPSC ★ FPSC

Microbes bring about biological transformation of xenobiotic compounds by

Question 19 of 33
★ PPSC ★ FPSC

Match the products in Group I with their producer organisms in Group II . Group I Group II P. Ethanol from glucose 1. Aspergillus niger Q. Probiotics 2. Leuconostoc mesenteroides R. Citric acid 3. Saccharomyces cerevisiae S. Sauerkraut 4. Bifidobacterium

Question 20 of 33
★ PPSC ★ FPSC

Identify the following antibiotics with their modes of action. Group I (Antibiotic) Group II (Mode of action) P. Ampicillin 1. Inhibition of protein synthesis Q. Tetracycline 2. Inhibition of cell wall synthesis R. Nystatin 3. Damage to cytoplasmic membrane S. Anthramycin 4. Damage to DNA structure

Question 21 of 33
★ PPSC ★ FPSC

Match the products in Group I with the microbial cultures in Group II used with their industrial production. Group I Group II P. Gluconic acid 1. Leuconostoc mesenteroides Q. L-Lysine 2. Aspergillus niger R. Dextran 3. Brevibacterium flavum S. Cellulase 4. Trichoderma reesei

Question 22 of 33
★ PPSC ★ FPSC

A biological process is involved in the . . . . . . . . treatment of industrial effluent.

Question 23 of 33
★ PPSC ★ FPSC

Which one of the following bioreactor configurations is the basis for a trickling biological filter?

Question 24 of 33
★ PPSC ★ FPSC

Ethanol concentration is lowest in

Question 25 of 33
★ PPSC ★ FPSC

The selection of the appropriate purification method in the product recovery after microbial fermentation depends on the

Question 26 of 33
★ PPSC ★ FPSC

Match items in Group I with correct examples from those in Group II . Group I Group II P. Catabolic product 1. Griseofulvin Q. Bioconversion 2. Baker's yeast R. Biosynthetic product 3. 6-Aminopenicillanic acid S. Cell mass 4. Ethanol

Question 27 of 33
★ PPSC ★ FPSC

Match the items in Group I with the terms given in Group II . Group I Group II P. Lactobacillus and Bifidobacteria 1. Prebiotics Q. Polychlorobenzenes (PCBs) 2. Probiotics R. Fructo-oligosaccharides 3. Antibiotics S. β-Lactams 4. Xenobiotics

Question 28 of 33
★ PPSC ★ FPSC

Enzyme papain is used with success to

Question 29 of 33
★ PPSC ★ FPSC

Which of the following is not true of aerobic digestion?

Question 30 of 33
★ PPSC ★ FPSC

Which one of the following options related to the following statements is incorrect? In comparison to physical/chemical methods of clean up, bioremediation methods

Question 31 of 33
★ PPSC ★ FPSC

Match the following products with their starting substrates. Group I Group II P. Sake 1. Apple juice Q. Cider 2. Grape juice c. Wine 3. Barley d. Lager 4. Rice

Question 32 of 33
★ PPSC ★ FPSC

A bacterial culture (200 μL containing 1.8 × 10 9 cells) was treated with an antibiotic Z (50 μg per mL) for 4 h at 37°C. After this treatment, the culture was divided into two equal aliquots. Set A: 100 μL was plated on Luria agar. Set B: 100 μL was centrifuged, the cell pellet washed and plated on Luria agar. After incubating these two plates for 24 h at 37°C, Set A plate showed no colonies, whereas the Set B plate showed 0.9 × 10 9 cells. This experiment showed that the antibiotic Z is

Question 33 of 33
★ PPSC ★ FPSC

A bioremedial solution to reduce oxides of nitrogen and carbon in flue gases is to integrate flue gas emission to

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In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.

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