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Microbiology In Biotechnology — Part 1 MCQs with Explanations

Practice high-yield Biotechnology Engineering MCQs on Microbiology In Biotechnology — Part 1 with worked academic solutions for PPSC, FPSC, NIH, NTS.

Questions
40
Difficulty Level
Exam-Level
Target Exams
PPSC, FPSC, NIH...
Estimated Time
48 mins
Exam-Level PPSC FPSC NIH NTS
Verified for Commission Syllabus • Oct 14, 2026
40

Verified Practice Questions

Interactive test mode with detailed academic worked solutions

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Question 1 of 40
★ PPSC ★ FPSC

In leguminous plants, both the rhizobium genes and the plant genes influence nodulation and nitrogen fixation. Which one of the following functions is not encoded by the host plant genes?

Question 2 of 40
★ PPSC ★ FPSC

Which one of the following organisms is used for the determination of phenol coefficient of a disinfectant?

Question 3 of 40
★ PPSC ★ FPSC

Match the cell structures in Group I with the organisms in Group II . Group I Group II P. Endospores 1. Methanobacterium Q. Bipolar flagella 2. Treponema R. Pseudomurine in cell wall 3. Spirillum S. Periplasmic flagella 4. Clostridium

Question 4 of 40
★ PPSC ★ FPSC

Match the human diseases in Group I with the causative agents in Group II . List-I List-II P. Amoebiasis 1. Leishmania donovani Q. African sleeping sickness 2. Trypanosoma cruzi R. Kala azar 3. Entamoeba histolytica S. Chagas' disease 4. Trypanosoma gambiense

Question 5 of 40
★ PPSC ★ FPSC

Which one of the following organisms is an indicator of fecal contamination?

Question 6 of 40
★ PPSC ★ FPSC

Match the antibiotics in Group I with their modes of action in Group II . Group I Group II P. Chloramphenicol 1. Inhibits protein synthesis by acting on 30S ribosomal subunit Q. Rifampicin Interferes with DNA replication by inhibiting DNA gyrase R. Tetracycline 3. Inhibits protein synthesis by acting on 50S ribosomal subunit S. Quinolone Interferes with RNA polymerase activity 5. Inhibits P-lactamase activity

Question 7 of 40
★ PPSC ★ FPSC

A list of pathogens (Group I) and a list of anti microbial agents (Group II) used to treat their infections are given below. Match the pathogens with the corresponding anti-microbial agents. Group I Group II P. Influenza A virus 1. Isoniazid Q. Fungus 2. Amantadine R. Plasmodium 3. Fluconazole S. Mycobacterium 4. Artemisinin 5. Iodoquinol

Question 8 of 40
★ PPSC ★ FPSC

Which of the following are true with regard to anaerobic respiration in bacteria? P. The final electron acceptor is an inorganic substance other than molecular oxygen. Q. The number of ATP molecules produced per glucose molecule is more than that produced in aerobic respiration. R. The number of ATP molecules produced per glucose molecule is less than that produced in aerobic respiration. S. Only substrate level phosphorylation is used to generate ATP.

Question 9 of 40
★ PPSC ★ FPSC

Which of the following are true about bacterial superoxide dismutase? P. Present in obligate aerobes. Q. Present in facultative anaerobes. R. Present in aerotolerant anaerobes. S. Absent in obligate aerobes.

Question 10 of 40
★ PPSC ★ FPSC

Match the drugs in Group I with their mechanism of action in Group II . Group I Group II P. Paclitaxel 1. Inhibits protein translation Q. Colchicine 2. Inhibits microtubule depolymerization R. Etoposide 3. Inhibits DNA replication S. Methotrexate 4. Alkylates DNA 5. Inhibits dihydrofolate reductase 6. Inhibits microtubule polymerization

Question 11 of 40
★ PPSC ★ FPSC

Match the products in Group I with their respective organisms in Group II . Group I Group II P. Glycerol 1. Corynebacterium glutamicum Q. Glutamic acid 2. Alcaligenes faecalis R. Curdlan 3. Dunaliella salina S. Amphotericin B 4. Streptomyces nodosus

Question 12 of 40
★ PPSC ★ FPSC

Coronavirus genome consists of

Question 13 of 40
★ PPSC ★ FPSC

Match the viruses in Group I with their host cell receptors in Group II . Group I Group II P. Hepatitis A virus 1. Heparan sulphate Q. Human immunodeficiency virus 2. Acetylcholine receptor R. Rabies virus 3. CD4 protein S. Herpes simplex virus 4. Alpha-2 macroglobulin

Question 14 of 40
★ PPSC ★ FPSC

Which one of the following does not belong to the domain of Bacteria?

Question 15 of 40
★ PPSC ★ FPSC

Which one of the following organisms is responsible for crown gall disease in plants?

Question 16 of 40
★ PPSC ★ FPSC

Tetracycline inhibits the

Question 17 of 40
★ PPSC ★ FPSC

Which one of the following is an example of a neurotoxin?

Question 18 of 40
★ PPSC ★ FPSC

Match Group I with Group II . List-I List-II P. Staphylococcus aureus 1. Biofilms Q. Candida albicans 2. Bacteriocins R. Mycobacterium tuberculosis 3. Methicillin resistance S. Lactobacillus lactis 4. Isoniazid

Question 19 of 40
★ PPSC ★ FPSC

Match the industrial products mentioned in Group I with their producer organisms in Group II . Group I Group II P. Citric acid 1. Trichoderma viride Q. Cellulase 2. Clostridium acetobutylicum R. Vitamin B 12 3. Aspergillus niger S. Butanol 4. Propionibacterium freudenreichii

Question 20 of 40
★ PPSC ★ FPSC

Match items in Group I with Group II . Group I Group II P. Alzheimer's disease 1. HINI Q. Mad cow disease 2. Hemoglobin R. Sickle cell anemia 3. Prions S. Swine flu 4. Amyloid

Question 21 of 40
★ PPSC ★ FPSC

Which one of the following is correct about microbial growth medium?

Question 22 of 40
★ PPSC ★ FPSC

Which of the following statements are correct? P. Viruses can play a role in causing human cancer. Q. A tumor suppressor gene can be turned off without any change in its DNA sequence. R. Alteration in miRNA expression levels contributes to the development of cancer.

Question 23 of 40
★ PPSC ★ FPSC

Determine the correctness or otherwise of the following Assertion (A) and Reason (R) . Assertion: Gram negative bacteria show staining with safranin. Reason: Gram negative bacteria have an outer membrane with lipopolysaccharides.

Question 24 of 40
★ PPSC ★ FPSC

Match the organisms in Group I with the characteristics in Group II . Group I Group II P. Methanococcus 1. Halophile Q. Dunaliella 2. Acidophile R. Sulfolobus 3. Mesophile S. Escherichia 4. Barophile

Question 25 of 40
★ PPSC ★ FPSC

Match the microorganisms in Group I with their fermentation products in Group II . Group I Group II P. Leuconostoc mesenteroides 1. Cobalamin Q. Rhizopus oryzae 2. Sorbose R. Gluconobacter suboxydans 3. Dextran S. Streptomyces olivaceus 4. Lactic acid 5. Butanol

Question 26 of 40
★ PPSC ★ FPSC

Diphtheria toxin, tetracycline and streptomycin inhibit

Question 27 of 40
★ PPSC ★ FPSC

Under stress conditions bacteria accumulate

Question 28 of 40
★ PPSC ★ FPSC

Match the compounds in Group I with the correct entries in Group II . Group I Group II P. Cyanide 1. K + ionophore Q. Antimycin A 2. Electron transfer from cytochrome b to cytochrome c 1 R. Valinomycin 3. F 1 subunit of ATP synthase S. Aurovertin 4. Cytochrome oxidase 5. Adenine nucleotide translocase

Question 29 of 40
★ PPSC ★ FPSC

Which combination of the following statements is correct for cyanobacteria? P. They can perform oxygenic photosynthesis. Q. Usually filamentous forms are involved in nitrogen fixation. R. Nitrogen fixation occurs in heterocyst. S. They cannot grow in a mineral medium exposed to light and air.

Question 30 of 40
★ PPSC ★ FPSC

A culture of bacteria is infected with bacteriophage at a multiplicity of 0.3. The probability of a single cell infected with 3 phages is

Question 31 of 40
★ PPSC ★ FPSC

The most plausible explanation for a sudden increase of the respiratory quotient (RQ) of a microbial culture is that

Question 32 of 40
★ PPSC ★ FPSC

Match the following antibiotics in Group I with their mode of action in Group II . Group I Group II P. Chloramphenicol 1. Binds to DNA gyrase Q. Norfloxacin 2. Binds to RNA Polymerase R. Puromycin 3. Inhibits peptidyl transferase S. Rifampicin 4. Mimics aminoacyl-tRNA

Question 33 of 40
★ PPSC ★ FPSC

The typical coenzyme present in the methanogens is

Question 34 of 40
★ PPSC ★ FPSC

Match the antibiotics in Group I with the targets in Group II . Group I Group II P. Sulfonamide 1. Peptidoglycan synthesis Q. Quinolones 2. Peptide chain elongation R. Erythromycin 3. Folic acid biosynthesis S. Cephalosporin 4. Topoisomerase

Question 35 of 40
★ PPSC ★ FPSC

A microorganism isolated from a salt-rich (salt concentration ∼2 M) lake was found to possess diglycerol tetraethers, with polyisoprenoid alcohol side chains, as the major lipid component of its cell membrane. The isolated organism is

Question 36 of 40
★ PPSC ★ FPSC

Bacteria with two or more flagella at one or both ends are called

Question 37 of 40
★ PPSC ★ FPSC

Which one of the following is not a product of denitrification in Pseudomonas?

Question 38 of 40
★ PPSC ★ FPSC

Which of the following are true for Treponema pallidum? P. It is the causative agent of syphilis Q. It is a spirochete R. It is a non-motile bacterium S. It is generally susceptible to penicillin Choose the correct combination.

Question 39 of 40
★ PPSC ★ FPSC

Match the organisms in Group I with the entries in Group II . Group I Group II P. Clostridium 1. Rods with teichoic acid in the cell wall Q. Escherichia 2. Rods with endospores R. Vibrio 3. Helical rods with flagella S. Bacillus 4. Rods with LPS in the outer membrane 5. Curved rods with polar flagella

Question 40 of 40
★ PPSC ★ FPSC

Which family of viruses has single stranded DNA?

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💡 Examiner's Advisory for Biotechnology Engineering

In competitive commission exams (FPSC, PPSC, KPPSC, SPSC, CSS, PMS), questions are frequently phrased with distractor options designed to catch rote learners. Whenever you answer an MCQ, read the Detailed Academic Explanation and Conceptual Anchor to understand why the remaining options are incorrect.

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