Molecular Tools In Biotechnology MCQs with Explanations

Fluorescent probe is the correct answer to “Expression of hundreds of different genes in DNA microarray technology is monitored by using”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Post-transcriptionally controlled is the correct answer to “Identical sized RNA transcript is detected by Northern blot analysis of UDP glucuronosyl transferase obtained from human liver and kidney. Microarray analysis of the same samples shows equal spot intensity, whereas Western blot detects a 55 kDa strong band in liver, but a very faint in kidney of same size. The regulation of UDP glucuronosyl transferase is”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

DNA microarray is the correct answer to “Which one of the following techniques/tools is not used for inserting a foreign gene into a cell”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

DNA microarray is the correct answer to “Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

2', 3'-dideoxy nucleoside triphosphate is the correct answer to “The nucleotide analogue used in DNA sequencing by chain termination method is”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Green fluorescent protein is the correct answer to “Expression of which of the following reporter genes do not require addition of specific substrate for detection”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

P-3, Q-1, R-2, S-4 is the correct answer to “Match the items in Group I with correct options in Group II . Group I Group II P. DNA footprinting 1. Protein-protein interaction Q. Yeast two-hybrid system 2. VNTR R. DNA fingerprinting 3. DNA binding protein S. SAGE 4. Transcriptome analysis”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Thermus thermophilus is the correct answer to “A thermostable DNA polymerase that can carry out both reverse transcription reaction and polymerization has been isolated form”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Primers is the correct answer to “A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg 2+ . Which one of the following is missing in the reaction mixture”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Taq DNA polymerase is the correct answer to “Enzyme used in 'cycle' sequencing of DNA is”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

NTPs is the correct answer to “Which one of the following is not a requirement for a PCR reaction”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Q and R only is the correct answer to “Which of the following statements about gene therapy are correct? P. Affected individuals, but not their progeny, can be cured through germline gene therapy. Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy. R. Affected individuals, but not their progeny, can be cured through somatic gene therapy. S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

P, Q and R is the correct answer to “Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction? P. Mg 2+ concentration Q. pH R. Annealing temperature”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

SNPs is the correct answer to “The heterozygosity of any locus can be ascertained by”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

ATCTTCTA and CCGCAAGC is the correct answer to “For PCR to amplify the sequence ATCTTCTACG . . . . . . . . AAGCPTGCGG TAGAAGATGC . . . . . . . .TTCGAACGCC The required primers are”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

< 1 is the correct answer to “In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

32% is the correct answer to “In human populations 4% of the individuals are homozygous recessive to a specific RFLP marker. What percentage of individuals does you expect to be heterozygous for this trait”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

SYBR green is the correct answer to “Which of the following fluorescent probes is used to monitor the progress of amplification in Real time PCR”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

P-3, Q-1, R-2, S-4 is the correct answer to “Match items in Group I with correct options from those given in Group II . Group I Group II P. VNTR sequence 1. Gene regulation on the same chromosome Q. Leader sequence 2. Ribosome binding site R. SD sequence 3. DNA fingerprinting S. cis-acting sequence 4. Functions in attenuation”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

5' CTGAGGT and 5' CCAACAT is the correct answer to “For the sequence of dsDNA given below, identify the set of primers required to amplify this DNA by PCR. 3' GACTCCA . . . . . . . . TACAACC 5' 5' CTGAGGT . . . . . . . . ATGTTGG 3'”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

P and S is the correct answer to “Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR). 5'-GACCTGTGG . . . . . . . . ATACGGGAT-3' 3'-CTGGACACC . . . . . . . . TATGCCCTA-5' Primers P. 5'-GACCTGTGG-3' Q. 5'-CCACAGGTC-3' R. 5'-TAGGGCATA-3' S. 5'-ATCCCGTAT-3'”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Foot printing is the correct answer to “Protein binding regions of DNA are identified by one of the following techniques.”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Primers is the correct answer to “Nick translation of DNA is a method for making DNA probes. Identify from below what is not required for nick translation method.”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

P-1, Q-3, R-5, S-4 is the correct answer to “Match the reagents in Group I with their preferred cleavage sites in Group II . Group I Group II P. Cyanogen bromide 1. Carboxyl side of methionine Q. o-Iodosobenzoate 2. Amino side of methionine R. Hydroxylamine 3. Carboxyl side of tryptophan S. 2-Nitro-5-thiocyanobenzoate 4. Amino side of cysteine 5. Asparagine-glycine bonds”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Size is the correct answer to “The mobility of DNA in agarose gel electrophoresis is solely based on its”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

4.19 × 105 is the correct answer to “A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Southern blotting is the correct answer to “A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

An electron microscope is used to gather data from the arrays is the correct answer to “All of the following are true about DNA microarray technology except”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

A partial sequence of a codon randomly selected from cDNA library is the correct answer to “Expressed Sequence Tag is defined as”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

In situ hybridization is the correct answer to “Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Adaptive immunity in prokaryotes is the correct answer to “CRISPR-Cas system is associated with”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

P and S only is the correct answer to “Identify the correct statements. P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction. Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid. R. T4 polynucleotide kinase is used for labeling 3' end of DNA. S. Baculovirus (Autographa californica) can be used as an insect expression vector.”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

\[S \cdot \cdot \cdot \cdot \cdot \cdot \cdot \cdot \xrightarrow{{Z4}}W\xrightarrow{{Z2}}X\xrightarrow{{Z1}}Y\xrightarrow{{Z3}}Z\] is the correct answer to “W, X and Y are the intermediates in a biochemical pathway as shown below: $$S \cdot \cdot \cdot \cdot \cdot \cdot \cdot \cdot \to W \to X \to Y \to Z$$ Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed): Mutants Media Supplemented with Mutants W X Y Z Z1 No No Yes Yes Z2 No Yes Yes Yes Z3 No No No Yes Z4 Yes Yes Yes Yes What is the order of the four complementation groups in terms of the step they block”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Betaine is the correct answer to “For amplification of GC rich sequences by polymerase chain reaction, identify the reagent that hinds and stabilizes AT sequences and destabilizes GC regions.”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Cut the DNA and hybridize with specific micro-satellite probes is the correct answer to “In order to identify the person who committed a crime, forensic experts will need to extract DNA from the tissue sample collected at the crime scene, and conduct one of the following procedures for DNA fingerprinting analysis”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Isoschizomers is the correct answer to “Restriction endonucleases from two different organisms that recognize the same DNA sequence for cleavage are called”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

Chromatin immunoprecipitation assay is the correct answer to “Protein-DNA interactions in vivo can be studied by”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

5' GCTAAGATCTGAATTTTC 3' and 5' GCGCTACATTATTGCCCA 3' is the correct answer to “The DNA sequence shown below is to be amplified by PCR: 5' GCTAAGATCTGAATTTTCC . . . . . . . . TTGGGCAATAATGTAGCGC 3' 3' CGATTCTAGACTTAAAAGG . . . . . . . . AACCCGTTATTACATCGCG 5' Which one of the following pair of primers can be used for this amplification”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.

DpnI is the correct answer to “For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA”. This question is standard in competitive examination syllabi for Molecular Tools In Biotechnology.