Recombinant Dna Technology — Part 2 MCQs with Explanations

Complementary DNA is the correct answer to “DNA synthesized from an RNA template is called”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Peptidyl tRNA hydrolase is the correct answer to “Which one of the following is not a protease”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Once every 4096 bases is the correct answer to “The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Double stranded DNA is the correct answer to “The substrate for restriction enzyme is”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

P and R only is the correct answer to “Hairy roots' induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by P. A high degree of lateral branching. Q. Genetic instability of culture. R. An absence of geotropism. S. Poor biomass production.”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

P-3, Q-1, R-4, S-2 is the correct answer to “Match the items in Group I with their functions in Group II . Group I Group II P. rol genes 1. Food and energy source Q. Opines 2. Tumor formation R. Virulence genes 3. Hairy root induction S. aux and cyt genes 4. T-DNA transfer and integration”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Lentivirus is the correct answer to “A vector derived from which one of the following viruses is used for high-frequency genomic integration of a transgene in animal cells”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

P and R only is the correct answer to “In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to P. Crown gall disease in plants. Q. Hairy root disease in plants. R. Transfer of T-DNA into the plant chromosome. S. Transfer of Ri-plasmid into the plant cell.”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Trimming single stranded regions in DNA is the correct answer to “Mung bean nuclease could be used for”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Bacterial artificial chromosome is the correct answer to “Human genome sequencing project involved the construction of genomic library in”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Ligation is the correct answer to “Which of the following processes require energy”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Inducible promoter is the correct answer to “A plasmid cloning vector should contain all of the following sequences except”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

EcoRI-2; BamHI-1; HindIII-2 is the correct answer to “"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments. Plasmid + HindIII → 1200bp and 3800bp Plasmid + BamHI → 5000bp Plasmid + EcoRI → 2500bp The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Both I and II are true is the correct answer to “Consider the following statements. I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase. II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Restriction endonucleases do not cut RNA, and this virus has an RNA genome is the correct answer to “You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

CMV promoter is the correct answer to “Which one of the following features is not required in a prokaryotic expression vector”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Large segments of DNA is the correct answer to “Yeast artificial chromosomes (YAC's) are used for cloning”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.

Polyhedrin gene is the correct answer to “In baculovirus expression vector, foreign genes are expressed from the promoter of”. This question is standard in competitive examination syllabi for Recombinant Dna Technology.